Lipase immobilization on ionic liquid‐modified magnetic nanoparticles: Ionic liquids controlled esters hydrolysis at oil–water interface |
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| Authors: | Yangyang Jiang Chen Guo Hongshuai Gao Hansong Xia Iram Mahmood Chunzhao Liu Huizhou Liu |
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| Affiliation: | 1. Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2. Research Institute of Nanjing Chemical Industrial Group, Nanjing 210048, China |
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| Abstract: | Ester hydrolysis at oil–water interface by lipase covalently immobilized on ionic liquid‐modified magnetic nanoparticles was investigated. Magnetic supports with a diameter of 10–15 nm were synthesized by covalent binding of ionic liquids (chain length C4 and C8 and anions Cl?, BF4?, and PF6?) on the surface of Fe3O4 nanoparticles. Lipase was covalently immobilized on Fe3O4 nanoparticles using ionic liquids as the coupling reagent. Ionic liquid‐modified magnetic nanoparticle‐grafted lipase preferentially located at the oil–water interface. It has higher catalytic activity than its native counterpart. A modified Michaelis–Menten model was used to elucidate the effect of stirring rate, aqueous–organic phase ratio, total amount of enzyme, and ester chain length. The influences of these conditions on esters hydrolysis at oil–water interface were consistent with the introduction of the ionic liquids interlayer. Ionic liquids could be used to control the oil–water interfacial characteristics during lipase catalyzed hydrolysis, and thus control the behavior of immobilized lipase. © 2011 American Institute of Chemical Engineers AIChE J, 2012 |
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| Keywords: | magnetic nanoparticles ionic liquids lipase oil– water two‐phase ester hydrolysis |
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