Ethanol‐Dispersed Polymer Nanofibers as a Highly Selective Cell Isolation and Release Platform for CD4+ T Lymphocytes |
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Authors: | Seung‐Hyun Jun Kwanghee Kim Hyo Jin An Byoung Chan Kim Chung Hee Sonn Miju Kim Junsang Doh Cassian Yee Kyung‐Mi Lee Jungbae Kim |
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Affiliation: | 1. Department of Chemical and Biological Engineering, Korea University, Seoul 136‐713, Republic of Korea;2. Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136‐713, Republic of Korea;3. Environment Sensor System Research Center, Korea Institute of Science and Technology, Seoul 136‐791, Republic of Korea;4. Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790‐784, Republic of Korea;5. School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790‐784, Republic of Korea;6. Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 USA |
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Abstract: | A novel cell isolation and release platform using electrospun polystyrene‐poly(styrene‐co‐maleic anhydride) (PS‐PSMA) nanofibers is presented. Ethanol treatment of PS‐PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter‐fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4+ T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4+ T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell‐supporting and releasing matrix. This is the first demonstration of lymphocyte‐culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4+ T cells for the purpose of cell therapies. |
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Keywords: | electrospun polymer nanofibers ethanol dispersion CD4+ T lymphocyte cell separation cell transplantation |
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