Supercritical Carbon Dioxide Extraction of <Emphasis Type="Italic">Microula sikkimensis</Emphasis> Seed Oil |
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Authors: | Shi Lina Ren Fei Zhao Xudong Du Yangong Han Fa |
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Affiliation: | (1) Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 59 Xiguan Street, Xining, 810001, Qinghai, People’s Republic of China;(2) Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100039, China;(3) QingHai Environmental Monitoring Center, 56 Gonghe Road, Xining, 810007, Qinghai, China |
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Abstract: | The seed oil of Microula sikkimensis had been intensively studied due to its pharmacological actions. In the present study, seed oil of Microula sikkimensis was extracted using supercritical fluid extraction (SFE). Determinations of the extracts composition were performed by gas
chromatography (GC). An orthogonal array design (OAD), OA9 (34), was employed for optimization of the supercritical fluid extraction of the compound with regard to the various parameters.
Four factors, namely pressure (21.0–27.0 MPa), the dynamic extraction time, temperature, and CO2 flow rate of the supercritical fluid, were studied and optimized by a three-level OAD. The effects of the parameters on the
yield of seed oil were studied using analysis of variance (ANOVA). The results revealed that the pressure had a significant
effect on the yield of seed oil (p < 0.05), while the other three factors, i.e., CO2 flow rate, dynamic extraction time and temperature, were not identified as significant factors under the selected conditions
based on ANOVA. The results show that the best values for the extraction condition of seed oil was pressure 24.0 MPa, extraction
time 3 h, temperature 45 °C and a CO2 flow rate 20 L/h in the 20-L vessel. |
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