~(60)Coγ射线照射对人血小板的影响

以不同剂量的~(60)Coγ射线照射富含血小板的血浆后,检测其代谢及释放产物的变化。~(60)Coγ射线照射剂量达5Gy时,血小板表面活化标记蛋白GMP-140分子的表达显著增多,且随剂量的增加而增高;但血小板膜糖蛋白Ⅰ_b和Ⅲ_a未见明显改变;当剂量为2.5Gy时血浆内血小板TXB_2的产生及释放就呈显著升高;γ射线剂量大于5Gy时,血浆内vWF的浓度显著升高。结果提示:~(60)Coγ射线照射剂量达5Gy后,可激活体外的血小板,导致后者代谢旺盛,内容物释放增多。

EFFECT OF ~(60)Co y-RAY IRRADIATION ON HUMAN PLATELETS

Human platelet-rich plasma was irradiated with various doses (0,1. 25,2. 5,5. 0, 10,20,40 Gy) of 60Co y-ray so as to observing the changes of metabolites and releasing substances of platelets. Alpha-granule membrane protein(GMP-140) molecules on the surface of platelet was expressed significantly increasing when the dosage of 60Co y-ray was 5 Gy; however, the glycoprotein (GP) Ib and la was not changed significantly ; thromboxane B2 production in plasma was significantly elevated while the y-ray was only 2. 5 Gy;the concentration of von Willebrand factor was increased when the y-ray was over 5 Gy,this is in accordance with the GMP-140 molecules. These results indicate that platelets could be activated in vitro when the dosage of 60Co y-ray was over 5 Gy. -^