多参考光合成孔径DMIPH术的细胞相位重构

为了克服传统光学显微术无法直接提取相位信息的不足而能更准确记录物体高频和低频信息的合成,采用多参考光合成孔径数字显微像面全息系统,并结合角谱算法和最小二乘解包裹算法实现了细胞的相位重构。选取活体细胞组织等相位型生物进行作为实验样本,对其进行定量观察和有效测量。结果表明,多参考光合成孔径数字显微像面全息系统可以有效地应用于3维物体显微结构的振幅和相位重构,能显著地提高记录系统的高频和低频信息在全息图上的合成度,并实现超出系统的衍射极限12.8lp/mm的分辨率。该系统可以有效地实现数字全息系统的超分辨率成像,从而获得细胞显微结构的3维形貌信息和准确的相位分布。

Reconstruction of cell phase by multi-reference light synthetic aperture digital microsopic image plane holographic technology

Digital holographic microscopy(DHM) extracts the phase information directly instead of traditional optical microscopy, and records high frequency and low frequency information of an object more accurately. Based on DHM, cell phase was reconstructed by means of angular spectrum algorithm and least-squares unwrapping algorithm. Quantitative observation and effective measurement were carried out for living biological sample cells. The experiment results indicates that multi-reference light synthetic aperture digital microscopic image plane holography can be effectively applied to the amplitude and phase reconstruction of the microstructures of three-dimension objects, it can improve synthetic degree of system significantly in recording the object’s high frequency and low frequency information, and the diffraction limit resolution 12.8lp/mm can be obtained. The results show that super resolution imaging of digital holographic can be effectively implemented based on this system, and three-dimension topography information and accurate phase distribution of the microstructure cells can be obtained.