A photoelectrocatalytic process that disinfects water contaminated with Mycobacterium kansasii and Mycobacterium avium |
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Authors: | Michelle Fernanda Brugnera Marcelo Miyata Guilherme Julião Zocolo Clarice Queico Fujimura Leite Maria Valnice Boldrin Zanoni |
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Affiliation: | 1. Department of Analytical Chemistry, Institute of Chemistry of Araraquara, UNESP, Rua Francisco Degni, 55 – Bairro Quitandinha, 14800-900 Araraquara, SP, Brazil;2. Department of Biological Science, Faculty of Pharmaceutical Science of Araraquara, UNESP, Rodovia Araraquara – Jaú, Km 01, 14801-902 Araraquara, SP, Brazil |
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Abstract: | Nontuberculous mycobacteria are resistant to conventional water treatment; indeed, they have been recovered from a wide variety of environmental sources. Here, we applied the photoelectrocatalytic technique using a Ti/TiO2–Ag photoanode to inactivate mycobacteria. For a mycobacteria population of 5 × 108 CFU mL−1, we achieved 99.9 and 99.8% inactivation of Mycobacterium kansasii and Mycobacterium avium with rate constant of 6.2 × 10−3 and 4.2 × 10−3 min−1, respectively, after 240 min. We compared the proposed method with the photolytic and photocatalytic methods. Using a mycobacteria population of 7.5 × 104 CFU mL−1, the proposed Ti/TiO2–Ag photoanode elicited total mycobacteria inactivation within 3 min of treatment; the presence of Ag nanoparticles in the electrode provided 1.5 larger degradation rate constant as compared with the Ti/TiO2 anode (1.75 × 10−2 for M. kansassi and 1.98 × 10−2 for M. avium). We monitored the degradation of the metabolites released during cellular lysis by TOC removal, sugar release, chromatography, and mass spectrometry measurements; photoelectrocatalysis and Ti/TiO2–Ag photoanodes furnished the best results. |
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Keywords: | Photoelectrocatalysis Disinfection TiO2 nanotubes Ag nanoparticles Mycobacteria |
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