| 柱前衍生化HPLC法分析牛奶中乳糖动态水解研究 |
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| 引用本文： | 丁海新,吴玉萍,肖伟洪,肖强.柱前衍生化HPLC法分析牛奶中乳糖动态水解研究[J].中国乳品工业,2006,34(12):48-50. |
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| 作者姓名： | 丁海新 吴玉萍 肖伟洪 肖强 |
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| 作者单位： | 1. 江西科技师范学院,江西省有机功能分子重点实验室,江西,南昌,330013
2. 江西教育学院,江西,南昌,330029 |
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| 基金项目： | 江西科技师范学院科研项目 |
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| 摘 要： | 采用1-苯基-3-甲基5-吡唑啉酮（PMP）对牛奶中的糖类进行衍生化处理，将其转化成紫外光下具有强吸收的物质．从而能利用高效液相法快速准确地监测牛奶中乳糖酶的动态水解趋势。结果表明，乳糖酶水解牛奶乳糖的最佳时间为3—4h．并且发现该方法可以直接用于乳糖含量的分析，为低乳糖奶制品的研究开发和检测分析提供参考。
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| 关 键 词： | 牛奶 乳糖酶 乳糖 PMP衍生化 |
| 文章编号： | 1001-2230（2006）12-0048-03 |
| 收稿时间： | 2006-06-08 |
| 修稿时间： | 2006-06-08 |
Analysis of lactose hydrolysis in milk by pre-column derivatization HPLC |
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| DING Hai-xin,WU Yu-ping,XIAO Wei-hong,XIAO Qiang.Analysis of lactose hydrolysis in milk by pre-column derivatization HPLC[J].China Dairy Industry,2006,34(12):48-50. |
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| Authors: | DING Hai-xin WU Yu-ping XIAO Wei-hong XIAO Qiang |
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| Affiliation: | 1.Jiangxi Key laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nangchang 330013, China; 2. Jiangxi Institute of education, Nangchang 330029, China |
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| Abstract: | A simple and sensitive pre-column derivatization method is described for the analysis of lactase hydrolysis in milk using high performance liquid chromatographic(HPLC).The 1-phenyl-3-methyl-5-pyrazolone(PMP) derivatives of Lactose,glucose and galactose were separated by reverse-phase HPLC using a gradient elution process,and monitored by ultraviolet detector at 245 nm.The result showed that the method could detect accurately the process of lactase hydrolysis.The optimal hydrolysis time is at 3-4 hours.On the other hand,the method could analyze reducing disaccharide directly,which may be useful for the analysis of sugars in dairy product. |
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| Keywords: | milk lactase lactose PMP derivatization |
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