2709碱性蛋白酶酶解鱼鳞工艺及其产物的功能特性

应用正交实验优选鱼鳞蛋白酶解的最佳条件。采用 2 70 9碱性蛋白酶 ,考察了酶量、固形物浓度、反应温度和酶解时间等因素对酶解反应的影响 ,确定最适条件。pH的选择参照了已有实验酶的最适 pH值 ,以水解度、游离氨基酸量为检测指标。结果表明 ,鱼鳞酶解宜采用碱性蛋白酶 2 70 9,用盐酸和氧化钙作软化的预处理 ,可使鱼鳞蛋白得到较高程度的水解 ,酶解最佳温度为 5 0℃ ,酶量宜采用 3 0 % ,酶解的最佳时间为 5h ,底物浓度宜选择 10 %。鱼鳞水解蛋白有很好的吸水性、乳化性、起泡性和溶解性 ;水解蛋白对羟基自由基也有很好的清除能力 ,其IC50 为 2 1mg/mL ,最大清除率为 93 6 %。

The Hydrolysis of Fish Scale and Its Properties

The hydrolysis conditions of fish scale using 2709 alkaline protease and founctional properties of the product were studied. The optimum conditions were found by mono-factor analysis and orthogonal test after fish scale was pre-treated with HCl and CaO, and the conditions are 10% concentration of the substrate, pH 8～9, 50℃ hydrolyzing temperature, 3% ratio of enzyme and substrate.The hydrolysate possesses good solubility, emulsifiability, and foaminess. The hydrolysate also showed good scavenging to·OH, with maximun scavenging rate reached 93.6%.