0.1 THz辐射对大鼠海马神经元兴奋性的影响

采用频率为0.1 THz、功率密度为2.65 mW/cm2的太赫兹光源分别辐射SD大鼠海马神经元5,15,25 min,通过神经元膜电位的变化,研究了太赫兹辐射对海马神经元兴奋性的影响,结果发现,15 min和25 min的太赫兹辐射会显著诱发海马神经元去极化,从而提高其兴奋性。为了探究太赫兹辐射提高神经元兴奋性的原因,检测了神经元内Ca^2+、Na^+和K+浓度的变化,结果表明,此辐射使海马神经元内Ca^2+、Na^+浓度增加,K+浓度减小。研究证实了太赫兹辐射(0.1 THz,2.65 mW/cm^2)通过调节海马神经元内带电离子的浓度促使其兴奋,为太赫兹辐射技术在生物医学领域应用的发展奠定了前期实验基础。

Effect of 0.1 THz Radiation on Excitability of Hippocampal Neurons in Sprague Dawley Rats

In this work, the effect of terahertz(THz) radiation on the excitability of hippocampal neurons is studied by changing the neuronal membrane potential and using hippocampal neurons in Sprague Dawley(SD) rats irradiated by a THz source with frequency of 0.1 THz and power density of 2.65 mW/cm2 for 5, 15, and 25 min, respectively. The results show that THz irradiation for 15 and 25 min causes a significant depolarization of the hippocampal neurons, thereby increasing neuron excitability. To explore the mechanism behind this THz radiation-induced excitability of neurons, the intracellular concentrations of Ca^2+, Na^+, and K^+ are determined. The results show that Ca^2+ and Na^+ concentrations in the hippocampal neurons increase and K^+ concentration decreases after irradiation by the THz source. Our study shows that the irradiation with frequency of 0.1 THz and power density of 2.65 mW/cm^2 can promote neuronal excitation by regulating the concentration of charged ions in the hippocampal neurons. This finding may provide the preliminary experimental basis for the application of THz radiation technology to the biomedical field.