气相色谱-质谱法测定化妆品中13种硝基苯类化合物

建立了水剂、粉剂、膏霜类、唇膏唇彩类化妆品中13种硝基苯类化合物的气相色谱-质谱（GC-MS）测定方法。水剂和粉剂类化妆品采用溶剂提取,浓缩后测定;膏霜类和唇膏唇彩类化妆品采用溶剂提取,佛罗里硅土固相萃取柱净化后测定。样液经OV-225（30m×0.25mm×0.25μm）毛细管色谱柱分离,GC-MS的选择离子监测（SIM）模式检测,以保留时间和特征离子比值定性,内标法定量。结果显示,13种硝基苯类化合物在0.1-5.0mg/L浓度范围内线性关系良好,方法检出限（S/N=3）在0.02-0.85mg/kg之间,3个添加水平的平均回收率在81.2%-11.3%之间,相对标准偏差（RSD,n=6）为4.5%-12.1%。

Simultaneous Determination of Thirteen Nitrobenzenes in Cosmetics by Gas Chromatography-Mass Spectrometry

A method to determine 13 nitrobenzens in cosmetics by gas chromatography-mass spectrometry（GC-MS）was established.Different pre-treatment methods were optimized according to the specific properties of different types of cosmetics.For powder and aqueous samples,directly GC-MS test could be operated after solvent extraction.For cream and lipstick,samples were extracted by appropriate solvent,followed by purification using Florisil solid phase extracting column.All of the samples were separated by OV-225（30m×0.25mm×0.25μm）chromatography column,and detected with selected ion monitoring（SIM）mode by GC-MS.The target analytes were qualitative confirmed by retention time and abundance ratio of characteristic ions,and quantified by internal standard method.Results showed that 13 nitrobenzens presented favorable linearity in a certain range of concentrations of 0.1 mg/L to 5.0 mg/L,the method detection limits ranged from 0.02 to 0.85 mg/kg（S/N=3）,the average recoveries ranged from 81.2% to 113% at three spiked levels,and the relative standard deviations（RSDs,n=6）were in the range of 4.5%to 12.1%.