不同辐照剂量对红豆分离蛋白结构及特性的影响

采用Lowery法、ANS荧光探针法、圆二色光谱、荧光光谱等方法分别对不同辐照处理的红豆分离蛋白溶解度、表面疏水性、蛋白质二级、三级结构、流体动力学半径进行分析。结果表明:随着辐照剂量的增加,红豆分离蛋白的二级结构中α-螺旋结构向无规则卷曲结构的转变,Trp残基所处微环境极性先增强后减弱,这与其表面疏水性呈现正相关,蛋白颗粒大小随着辐照剂量的增加先减小后增大,在5 k Gy辐照剂量处理时达到最小,这与其溶解性呈现负相关。

Different Iradiation Dose on the Structure and Functional Effects of Red Bean Protein Isolates

The study is to analyze the differences of structure and functional property of red bean protein isolates which treated with different irradiation dose, by using Lowery, ANS fluorescence probe method, circular dichroism and fluorescence spectrum. First of all,the results show that the alpha helix structure was changed into random coil, with the increase of irradiation dose.Secondly,the micro environment polarity of Trp residue increased first and then decreased,which indicated the positive correlation with surface hydrophobicity. Thirdly, protein particle size increase with the decrease of the first and reduced to the minimum,when the irradiation dose is 5kGy.And the result of protein particle size had negative correlation with the solubility of red bean protein isolates.