| 原子吸收光谱法间接测定樟树叶片中叶绿素总量 |
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| 引用本文： | 徐绍炳,吴一微,郑颖.原子吸收光谱法间接测定樟树叶片中叶绿素总量[J].福建分析测试,2007,16(2):5-8. |
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| 作者姓名： | 徐绍炳 吴一微 郑颖 |
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| 作者单位： | 湖北师范学院化学与环境工程系,湖北黄石,435002 |
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| 摘 要： | 探讨了樟树叶片中叶绿素提取的条件,优化了原子吸收光谱仪的测定参数,建立了用无水乙醇作提取剂在研磨条件下提取叶绿素、用石油醚萃取以分离出有机镁、用0.8mol/L盐酸反萃取,使镁进入水溶液。采用火焰原子吸收分光光度法测定萃取液中的镁含量,从而间接测定樟树叶片中叶绿素的含量的分析方法。结果表明,方法的回收率为103~116%,相对标准偏差为4.8%。
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| 关 键 词： | 叶绿素 镁 间接测定 原子吸收分光光度法 |
| 文章编号： | 1009-8143（2007）02-0005-04 |
| 修稿时间： | 2006-06-27 |
Indirect determination of chlorophyll in paint by atomic absorption Spectrometry |
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| Xu Shaobing,Wu Yi wei,Zheng Ying.Indirect determination of chlorophyll in paint by atomic absorption Spectrometry[J].Fujian Anal Ysis & Testing,2007,16(2):5-8. |
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| Authors: | Xu Shaobing Wu Yi wei Zheng Ying |
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| Affiliation: | Department of Chemistry and environmental Engineering, Hubei Normal University, huangshi, HuBei 435002, China |
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| Abstract: | The conditions of the extraction of chlorophyll from the leaf were discussied in this study,and the parameters of determination by flame atomic absorption Spectrometry were optimized.A method for the indirect determination of chlorophyll was established.The pretreatment plant leaf was pulverized with absolute alcohol to extract chlorophyll from the leaf,and further extracted with petroleum ether to separate the organic magnesium from the alcoholic-extraction solution.Then the organic phase was the back-extracted with 0.8mol/L hydrochloric acid,free Mg2 ion was finally determined by flame atomic absorption spectrometry,and the content of chlorophyll was determinated indirectly.The recovery was from 103~116%,and RSD was 4.8%. |
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| Keywords: | chlorophyll magnesium atomic absorption spectrometry(AAS) indirect determination |
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