| 2018—2020年高邮市小肠结肠炎耶尔森菌流行病学及病原学特征分析 |
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| 引用本文： | 刘文俊,周 信,高金彬,孙 翔,崔仑标,周 乐,徐娅雯,王 艳,徐 勤.2018—2020年高邮市小肠结肠炎耶尔森菌流行病学及病原学特征分析[J].食品安全质量检测技术,2021,12(23):9286-9291. |
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| 作者姓名： | 刘文俊 周 信 高金彬 孙 翔 崔仑标 周 乐 徐娅雯 王 艳 徐 勤 |
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| 作者单位： | 扬州市疾病预防控制中心,扬州市疾病预防控制中心,高邮市疾病预防控制中心,高邮市疾病预防控制中心,江苏省疾病预防控制中心,扬州市疾病预防控制中心,扬州市疾病预防控制中心,扬州市疾病预防控制中心,扬州市疾病预防控制中心 |
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| 基金项目： | 国家卫生健康委员会肠道病原微生物重点实验室(江苏省疾病预防控制中心)开放基金课题(EM201903)；江苏省卫生健康委员会医学科研项目(Z2020050)；扬州市科技计划社会发展项目(YZ2020122)。 |
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| 摘 要： | 目的 了解高邮市小肠结肠炎耶尔森菌流行病学及病原学特征，为本地区该菌的防控提供科学依据。方法 2018-2020年在高邮市采集腹泻患者粪便、家畜家禽粪便、苍蝇及生猪屠宰场组织样本等各类样本共计2682份。采用冷增菌方法进行小肠结肠炎耶尔森菌的分离培养，对分离菌株进行生化鉴定、血清分型、生物分型和毒力基因的荧光PCR检测。结果 2682份样本中共检出小肠结肠炎耶尔森菌120株，检出率为4.47%，包括猪粪源菌株99株，鸭粪源菌株2株，苍蝇源菌株4株，生猪屠宰场源菌株15株。分离菌株血清型以O:3型为主，生物型主要为3型，毒力基因检测结果显示，具有致病性的分离菌株共73株，其中O:3血清型69株、O:9血清型1株和血清未定型3株，且以猪粪源菌株为主。结论 小肠结肠炎耶尔森菌在高邮市畜禽动物、苍蝇媒介及生猪屠宰场中存在流行分布，猪的检出率最高，且致病性菌株携带率最高，主要致病型别为生物3型/ O:3血清型（3/ O:3型）。
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| 关 键 词： | 小肠结肠炎耶尔森菌 血清型 生物型 毒力基因 |
| 收稿时间： | 2021/9/2 0:00:00 |
| 修稿时间： | 2021/12/7 0:00:00 |
Analysis of epidemiological and etiological characteristics of Yersinia enterocolitica in Gaoyou city from 2018 to 2020 |
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| LIU Wen-Jun,ZHOU Xin,GAO Jin-Bin,SUN Xiang,CUI Lun-Biao,ZHOU Le,XU Ya-Wen,WANG Yan,XU Qin.Analysis of epidemiological and etiological characteristics of Yersinia enterocolitica in Gaoyou city from 2018 to 2020[J].Food Safety and Quality Detection Technology,2021,12(23):9286-9291. |
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| Authors: | LIU Wen-Jun ZHOU Xin GAO Jin-Bin SUN Xiang CUI Lun-Biao ZHOU Le XU Ya-Wen WANG Yan XU Qin |
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| Affiliation: | Yangzhou Center for Disease Control and Prevention,Yangzhou Center for Disease Control and Prevention,Gaoyou Center for Disease Control and Prevention,Gaoyou Center for Disease Control and Prevention,Jiangsu Center for Disease Control and Prevention,Yangzhou Center for Disease Control and Prevention,Yangzhou Center for Disease Control and Prevention,Yangzhou Center for Disease Control and Prevention,Yangzhou Center for Disease Control and Prevention |
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| Abstract: | Objective To understand the epidemiological and etiological characteristics of Yersinia enterocolitica in Gaoyou, which provided the basis for the prevention and control of Yersinia enterocolitica in local area. Methods A total of 2682 samples, including stool specimens from patients with diarrhea and domestic animals, flies and pig tissue samples from local slaughter houses, were collected in Gaoyou between 2018 and 2020. Cold enrichment method was used to isolate Yersinia enterocolitica. The isolated strains were analyzed by biochemical identification, serotyping, biotyping and virulence gene detection with fluorescence quantitative PCR. Results There were 120 strains of Yersinia Enterocolitica isolated from the 2682 specimens with a detection rate of 4.47%, including 99 strains from pig feces, 2 strains from duke feces, 4 strains from flies and 15 strains from pig slaughter houses. The predominant serotype was O:3 and the biotypes of the strains were mainly 3. Results of the virulence gene analysis showed that 73 strains were pathogenic, including 69 strains of O:3, 1 strain of O:9 and 3 strains of undetermined serotype, mainly from pig strains. Conclusion Distribution of Yersinia Enterocolitica existed in domestic animals, flies and pig slaughter houses in Gaoyou City. The detection rate of Yersinia Enterocolitica was the highest in pigs, and the carrying rate of pathogenic strains was the highest. The dominant pathogenic strains were bio-serotype 3/ O:3. |
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| Keywords: | Yersinia Enterocolitica Serotype Biotype Virulence genes |
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