The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase |
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| Authors: | R Cantoni M Branzoni M Labò M Rizzi G Riccardi |
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| Affiliation: | Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy. |
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| Abstract: | The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase. |
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