基于高通量测序技术奶豆腐核心细菌类群的研究

使用MiSeq高通量测序技术对15个奶豆腐样品中细菌多样性进行了解析。结果表明,新疆维吾尔自治区塔城地区奶豆腐中优势细菌门为Proteobacteria(变形菌门)、Frimicutes(硬壁菌门)、Actinobacteria(放线菌门)和Bacteroidetes(拟杆菌门)。在所有样品中均存在且相对含量大于1.0%的细菌属为Lactobacillus(乳杆菌属,19.66%)、Acetobacter(醋酸杆菌属,16.69%)、Lactococcus(乳球菌属,13.90%)、Enterobacter(肠杆菌属,11.42%)、Staphylococcus(葡萄球菌属,3.33%)、Acinetobacter(不动杆菌属,2.79%)和Streptococcus(链球菌属,1.14%)。核心分类操作单元矩阵OTU注释为乳酸杆菌属、Macrococcus(巨球菌属)、乳球菌属、肠杆菌属、不动杆菌属和醋酸杆菌属。由此可见,塔城地区奶豆腐中含有大量的细菌类群,且以乳杆菌属、醋酸杆菌属、乳球菌属和肠杆菌属细菌为主。

Study on the Core Bacterial Microbiota of Dairy Toufu Based on High-throughput Sequencing Technology

This study used MiSeq high-throughput sequencing technology to analyze the bacterial diversity in 15 dairy toufu samples.The results showed that the dominant bacteria in dairy toufu in Tacheng area of Xinjiang Uygur Autonomous Region were Proteobacteria,Frimicutes,Actinobacteria and Bacteroidetes.The bacterial genus presented in all samples and with the relative abundance more than 1.0%were Lactobacillus(19.66%),Acetobacter(16.69%),Lactococcus(13.90%),Enterobacter(11.42%),Staphylococcus(3.33%),Acinetobacter(2.79%)and Streptococcus(1.14%).The core operational taxonomic units were identified as Lactobacillus,Macrorococcus,Lactococcus,Enterobacter,Acinetobacter and Acetobacter.It can be seen that the dairy toufu in Tacheng area contains a large number of bacterial groups,and was mainly composed of Lactobacillus,Acetobacter,Lactococcus and Enterobacter.