3D打印医用钛合金的抗菌性能和体外生物相容性

应用选择性激光熔融技术(SLM)制备出3D打印医用钛合金Ti-6Al-4V和Ti-6Al-4V-5Cu，用平板共培养法研究测定其抗菌性能，用CCK8细胞增殖测定法、鬼笔环肽细胞骨架染色法和Annexin-V/PI流式细胞术研究了这种合金的抗菌性能和对小鼠胚胎成骨前体细胞(MC3T3-E1)的体外生物相容性影响。结果表明，3D打印Ti-6Al-4V-5Cu合金具有较高的抗菌性能，对金黄色葡萄球菌的抗菌率达到(57.03±1.55)%。在CCK8细胞增殖毒性测定、细胞骨架鬼笔环肽染色实验和Annexin-V/PI双标记法流式分析三种研究中Ti-6Al-4V-5Cu表现的优越，具有更好的体外生物相容性。

Antibacterial Properties and Biocompatibility of SLM-fabricated Medical Titanium Alloys

The 3D printing medical titanium alloys Ti-6Al-4V and Ti-6Al-4V-5Cu were prepared by selective laser melting technology (SLM), and their antibacterial properties were assessed by plate co-culture method. The in vitro biocompatibility with the mouse embryonic osteogenic precursor cells (MC3T3-E1) of the prepared alloys was systematically investigated by means of methods of CCK8 cell proliferation assay, phalloidin cytoskeleton staining and Annexin-V/PI flow cytometry. The results show that the 3D printing Ti-6Al-4V-5Cu alloy has high antibacterial property and the antibacterial rate against Staphylococcus aureus is 57.03%. The alloy Ti-6Al-4V-5Cu performed well with better in vitro biocompatibility during the three assessments,namely,CCK8 cell proliferation toxicity assay, cytoskeleton phalloidin staining experiment and Annexin-V/PI double labeling flow analysis.