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Re‐endothelialization of Human Umbilical Arteries Treated with Polyelectrolyte Multilayers: A Tool for Damaged Vessel Replacement |
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| Authors: | H. Kerdjoudj C. Boura V. Moby K. Montagne P. Schaaf J.‐C. Voegel J.‐F. Stoltz P. Menu |
| Affiliation: | 1. Group of Bioengineering (LEMTA‐UMR CNRS 7563), School of Medicine, Nancy University, 54505 Vandoeuvre‐lès‐Nancy (France);2. Department of Pathology, H?pital de Brabois Adultes, Rue du Morvan, 54511 Vandoeuvre (France);3. Charles Sadron Institut (UPR 22, CNRS), 6, Rue Boussingault, 67083 Strasbourg Cedex (France);4. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 595, 11, Rue Humann, 67085 Strasbourg Cedex (France);5. Odontology faculty, Louis Pasteur University, 1, Place de L'h?pital, 67000 Strasbourg (France) |
| Abstract: | The use of cryopreserved arteries for vascular tissue engineering provides a promising way for vessel replacement. Unfortunately cryopreservation induces structural changes that strongly modify the mechanical properties and alter the thrombogenicity of the vessel after implantation. We present here a new procedure to treat the inner coating of cryopreserved arteries with poly(sodium‐4‐styrene sulfonate)/poly(allylamine hydrochloride) polyelectrolyte multilayers. We show that this treatment improves the mechanical properties of the cryopreserved vessel. It also allows the adhesion and spreading of endothelial cells so that the internal structure of the vessel closely resembles that of fresh arteries. Finally, we verify by PECAM‐1 and von‐Willebrand‐factor (vWF) expression that this treatment preserves the phenotype of the endothelial cells. This study should open new routes towards the development of future, new biocompatible tissue substitutes allowing long‐term functionality after implantation. |
| Keywords: | Biocompatible materials Polyelectrolytes Structure– property relationships Tissue engineering |