| 分散液液微萃取-气相色谱-质谱分析尿样中的氯氮卓 |
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| 引用本文： | 李谈,周娜,梁玲琳,周海梅,齐孝蕊.分散液液微萃取-气相色谱-质谱分析尿样中的氯氮卓[J].福建分析测试,2012(5):38-40. |
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| 作者姓名： | 李谈 周娜 梁玲琳 周海梅 齐孝蕊 |
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| 作者单位： | 河南科技大学法医学院,河南洛阳471003 |
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| 基金项目： | 河南科技大学SRTP项目(编号:2010078) |
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| 摘 要： | 目的:用分散液液微萃取-气相色谱-质谱联用技术提取检测生物检材中的氯氮卓。方法:以氯仿为微萃取剂,甲醇为分散剂,在pH8提取尿样中的氯氮卓,气相色谱-质谱法分析,用内标标准曲线法测定氯氮卓的含量。结果:尿样中氯氮卓的线性范围为0.01～2μg/ml,检出限为0.002?g/ml;方法回收率为87.3%,RSD=3.47%(n=5)。结论:该方法操作简便、灵敏度高,适于尿样中氯氮卓的快速分析。
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| 关 键 词： | 氯氮卓 分散液相微萃取 气相色谱质谱联用 尿 |
Analysis of Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry |
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| Li Tan,Zhou Na,Liang Ling-lin,Zhou Hai-mei,Qi Xiao-rui.Analysis of Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry[J].Fujian Anal Ysis & Testing,2012(5):38-40. |
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| Authors: | Li Tan Zhou Na Liang Ling-lin Zhou Hai-mei Qi Xiao-rui |
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| Affiliation: | (Faculty of Forensic Medicine,Henan University of Science and Technology,Luoyang,Henan 471003,China) |
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| Abstract: | Objective： To detect Chlordiazepoxide in urine by dispersive liquid-liquid micro-extraction and gas chromatography mass spectrometry. Methods： Chlordiazepoxide in urine was extracted with chloroform and methanol at pH8 and was analyzed by GC-MS, its content was determined with internal standard curve. Results： The detection limit of the Chlordiazepoxide in urine was 0.002μ g/ml, and the standard curve was linear in the range of 0.01 -2 μ g/ml. The average recovery of Chlordiazepoxide was 89.8% （RSD = 3.86%, n = 5 ）. Conclusion： This method is simple, high sensitivity and suitable for quick analysis of the Chlordiazepoxide in urine. |
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| Keywords: | Chlordiazepoxide dispersive liquid-liquid micro-extraction gas chromatography mass spectrometry urine |
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