| 茶树菇多糖的提取工艺 |
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| 引用本文： | 邓超,邬敏辰. 茶树菇多糖的提取工艺[J]. 食品与发酵工业, 2006, 32(1): 118-121 |
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| 作者姓名： | 邓超 邬敏辰 |
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| 作者单位： | 江南大学医学系,江苏,无锡,214000 |
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| 摘 要： | 对茶树菇胞内和胞外多糖的提取工艺进行了研究,通过单因素法确定了胞内多糖水提法的最适条件:提取温度80~90℃,水料体积比为4∶1,提取10 h,共提取3次,醇析中加入3倍体积的体积分数为95%的乙醇。应用响应面分析法对胞外多糖醇析过程进行了优化。结果表明,在添加4倍体积的体积分数为95%乙醇,醇析17 h,pH5.5的条件下,胞外多糖产量可达5.76 mg/mL。
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| 关 键 词： | 茶树菇多糖 提取工艺 响应面分析 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-21 |
The Extractive Technique of Agrocybe Aegirit Polysaccharide |
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| Deng Chao,Wu Minchen. The Extractive Technique of Agrocybe Aegirit Polysaccharide[J]. Food and Fermentation Industries, 2006, 32(1): 118-121 |
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| Authors: | Deng Chao Wu Minchen |
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| Affiliation: | Department of Medicine, Southern Yangtze University, Wuxi 214000,China |
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| Abstract: | In this paper,we investigated the effects of various conditions of extraction on the polysaccharides of Agrocybe aegirit.The optimum extractive condition of intracellular polysaccharides was determined through single factor test as follows:extracting temperature 80℃～90℃;water:mycelia ratio 4∶1;extracting time is 10 hours;extracting in 3 times;precipitated by addition of 3 volumes of 95% ethanol.Optimized process of precipitated by ethanol for extracellular polysaccharides through response surface analysis.The results indicated that with addition of 4 volumes of 95% ethanol,precipitated 17 h,pH5.5 conditions,the yield of extracellular polysaccharides was about 5.76 mg/mL. |
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| Keywords: | Agrocybe aegirit polysaccharides extractive technique response surface analysis |
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