| 一种检测环境样品和细胞中肼的香豆素类比率型荧光探针 |
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| 引用本文： | 盛筱,沈亚龙,张晨,张艺雯,刘树玲,王守信. 一种检测环境样品和细胞中肼的香豆素类比率型荧光探针[J]. 精细化工, 2023, 40(12) |
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| 作者姓名： | 盛筱 沈亚龙 张晨 张艺雯 刘树玲 王守信 |
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| 作者单位： | 济宁医学院药学院,济宁医学院药学院,济宁医学院药学院,济宁医学院药学院,济宁医学院药学院,济宁医学院药学院 |
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| 基金项目： | 山东省自然科学基金项目(ZR2020MB107)；山东省大学生创新创业训练计划项目(S202210443009) |
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| 摘 要： | 以香豆素为荧光团,4-溴丁酰基为识别基团,设计合成了一种比率型肼荧光探针COCB。其结构通过1HNMR、13CNMR和HRMS确证。肼对探针COCB中溴代丁酰基的选择性脱保护使分子内电荷转移(ICT)过程恢复；COCB在 420 nm 处蓝色荧光衰减,而在 480 nm 处青色荧光增强,实现了对肼的比率检测。COCB对肼表现出高选择性、高灵敏度和强抗干扰能力,并能在较宽的线性范围(0~250 μmol/L)和pH范围(6~11)内检测肼,检出限低至0.15μmol/L。此外,COCB合成简便,细胞毒性较低,已成功用于实际水样、土壤以及活细胞中肼的检测。
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| 关 键 词： | 香豆素 肼 荧光探针 比率型 细胞成像 功能材料 |
| 收稿时间： | 2023-01-13 |
| 修稿时间： | 2023-03-22 |
A Coumarin-Based Ratiometric Fluorescent Probe for Detection of Hydrazine in Environmental Samples and Living Cells |
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| SHENG Xiao,SHEN Yalong,ZHANG Chen,ZHANG Yiwen,LIU Shuling and WANG Shouxin. A Coumarin-Based Ratiometric Fluorescent Probe for Detection of Hydrazine in Environmental Samples and Living Cells[J]. Fine Chemicals, 2023, 40(12) |
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| Authors: | SHENG Xiao SHEN Yalong ZHANG Chen ZHANG Yiwen LIU Shuling WANG Shouxin |
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| Affiliation: | School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China,School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China,School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China,School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China,School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China,School of Pharmaceutical Sciences,Jining Medical University,Rizhao,276826;China |
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| Abstract: | |
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| Keywords: | coumarin hydrazine fluorescent probe ratiometric cell imaging functional materials |
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