绿豆皮黄酮的提取纯化及其抗氧化研究 |
| |
引用本文: | 罗磊,姬青华,朱文学,焦昆鹏,关宁宁,薛依涵. 绿豆皮黄酮的提取纯化及其抗氧化研究[J]. 中国粮油学报, 2017, 32(9): 109 |
| |
作者姓名: | 罗磊 姬青华 朱文学 焦昆鹏 关宁宁 薛依涵 |
| |
作者单位: | 河南科技大学食品与生物工程学院,河南科技大学食品与生物工程学院,河南科技大学食品与生物工程学院,河南科技大学食品与生物工程学院,河南科技大学食品与生物工程学院,河南科技大学食品与生物工程学院 |
| |
基金项目: | 国家自然基金联合基金项目(U1304330) |
| |
摘 要: | 本研究对绿豆皮黄酮微波辅助提取、大孔吸附树脂纯化及其体外抗氧化活性进行研究。结果表明,当绿豆皮黄酮的微波辅助提取条件为液料比35∶1,微波时间80 s,微波功率540 W,乙醇体积分数70%时,绿豆皮黄酮的提取量达到8.467 mg/g。15种树脂的吸附和解吸动力学研究结果表明,NKA-9型大孔吸附树脂对绿豆皮黄酮有较好的纯化效果,纯化后黄酮的纯度由37.14%提高到71.08%,黄酮回收率可达82.8%。抗氧化活性试验表明,绿豆皮黄酮清除超氧阴离子自由基的能力与作用时间成反比,与提取液质量浓度成正比;绿豆皮黄酮对1,1-二苯基-2-三硝基苯肼自由基和·OH自由基有较好的清除能力,经NKA-9树脂初步纯化后,其抗氧化能力显著提高。
|
关 键 词: | 绿豆皮;黄酮;提取;纯化;抗氧化 |
收稿时间: | 2016-08-18 |
修稿时间: | 2017-01-17 |
Study on Extraction purification and Antioxidation of Flavonoids from Mung Bean Hull |
| |
Abstract: | Abstract Study on microwave-assisted extraction, macroporous resin purification and in vitro antioxidant activity of flavonoids from mung bean hull. The results showed that the optinal extraction parameters were as follow : liquid-to-solid ratio, 35: 1, microwave time , 80s, microwave power, 540 w, ethanol volume fraction,70%,leading to the maximn extraction yield of 8.467 mg/g. The results of the 15 kinds of resin adsorption and desorption kinetics studies showed that NKA-9 resin revealed a good ability to purification flavonoids from mung bean hull. After the purification ,the purity of flavonoids from mung bean hull was increased from 37.14% to 71.08%,the recovery of flavonoids reached up to 82.8%.Antioxidant activity tests of the flavonoids from mung bean hull showed that its ability of scavenging effects on superoxide action free radical is inversely proportional to the time, while it become direct ratio get along with consistence. It has a better scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and the hydroxyl radical. After purification, antioxidant activities were improved significantly than before. |
| |
Keywords: | Mung Bean Hull flavonoids extraction purification antioxidant activities |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《中国粮油学报》浏览原始摘要信息 |
|
点击此处可从《中国粮油学报》下载全文 |
|