Distribution of dietary octadecenoate isomers at the 1- and 2-positions of hepatoma and liver phospholipids |
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Authors: | Randall Wood Fred Chumbler |
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Affiliation: | (1) Department of Biochemistry and Biophysics, Texas Agricultural Experiment Station, Texas A&M University System, 77843 College Station, Texas |
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Abstract: | Phosphatidylcholines and phosphatidylethanolamines were isolated from hepatoma 7288CTC, normal liver, and host liver of rats
fed one of the following diets: fat-free diet; fat-free diet supplemented with safflower oil, safflower oil fatty acids, or
partially hydrogenated safflower oil fatty acids; and commercial chow. Thecis andtrans octadecenoate fatty acids were isolated from the 1- and 2-positions of both phosphoglycerides and analyzed quantitatively
for chain positional isomers. Octadecenoates from hepatoma and liver phosphoglycerides of animals fed fat-free or natural
fatsupplemented diets contained almost exclusively twocis isomers: oleic and vaccenic acids. Oleic acid predominated in the 2-position octadecenoates of both phosphoglycerides from
hepatoma and liver. In contrast, vaccenic acid predominated in the 1-position of normal liver phosphatidylcholine and, to
a lesser extent, phosphatidylethanolamine. Host liver and hepatoma exhibited a shift to a higher percentage of oleic acid
at the 1-position. Dietarytrans fatty acids were incorporated predominately in the 1-position of both phosphoglycerides of hepatoma and liver. Except for
thecis Δ10 octadecenoate isomer, all of the unnatural dietarycis isomers between Δ8 and Δ14 were incorporated into the 1-position of the phospholipids, while the unnaturalcis octadecenoates at the 2-position consisted primarily of the Δ12 isomer. Hepatoma phosphoglycerides contained higher percentages
of thetrans Δ10 isomer that was nearly excluded from the 1-position of the two liver phosphoglycerides. All the othertrans octadecenoate isomers were incorporated into the 1-position of both phosphoglycerides, but the small amount oftrans fatty acids incorporated into the 2-position of liver and hepatoma phosphatidylcholine consisted of four isomers, Δ9 to Δ12,
including the Δ10 isomer. Phosphatidylethanolamine exhibited a similar distribution, except for the presence of the Δ13 and
Δ14 isomers at the 2-position. A combination of evidence suggests that the 1-position fatty acids in phosphatidylcholine and
phosphatidylethanolamine are of similar origin. The octadecenoates at the 2-position of these two phosphoglycerides appear
to be of the same origin in hepatoma but not in liver. It was also revealed that the 2-position of hepatoma phosphatidylcholine
contained much higher percentages of palmitate than liver. |
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