Isolation of powerful antioxidants from the medicinal halophyte Limoniastrum guyonianum |
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Authors: | Trabelsi Najla Oueslati Samia Falleh Hanen Waffo-Téguo Pierre Papastamoulis Yorgos Mérillon Jean-Michel Abdelly Chedly Ksouri Riadh |
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Affiliation: | Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia; Laboratoire de Sciences Végétales, Mycologie et Biotechnologie, Groupe d'Etude des Substances Végétales à Activité Biologique (GESVAB) EA 3675 - UFR Pharmacie, Institut des Sciences de la Vigne et du Vin (ISVV), Université Victor Segalen Bordeaux 2, France. |
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Abstract: | The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of Limoniastrum guyonianum Boiss. (Zita) were investigated in this study. The ethyl acetate phase exhibited a significant antioxidant activity as judged by total antioxidant activity, DPPH test and reducing power. Fractionation of this extract by centrifugal partition chromatography (CPC) using quaternary Arizona solvent systems composed of n-heptane/ethyl acetate/methanol/water led to ten fractions. The antioxidant capacities of these fractions were assessed using the same previous tests. Fraction 8 showed the highest antioxidant capacity (1291.1mg GAE/g DR), the power ability to quench DPPH radical (IC(50)=2μg/ml) and to reduce Fe(3+) (EC(50)=65μg/ml). From this fraction, three powerful flavonoids were isolated (1-3): gallocatechin (1), epigallocatechin (2) and epigallocatechin-3-O-gallate (3). These findings suggest that the antioxidative property of L. guynianum is may be related to the presence of these flavonoids, which can be used in various industrial fields. |
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Keywords: | DPPH, 2,2-diphenyl-1-picrylhydrazyl IC50, Inhibition Concentration at 50 percent EC50, effective concentration at which the absorbance was 0.5 GAE, gallic acid equivalents BHT, butylated hydroxytoluene BHA, butylated hydroxyanisole TFA, trifluoroacetic acid MeCN, acetonitrile |
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