Degradation of white wine haze proteins by Aspergillopepsin I and II during juice flash pasteurization |
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Authors: | Marangon Matteo Van Sluyter Steven C Robinson Ella M C Muhlack Richard A Holt Helen E Haynes Paul A Godden Peter W Smith Paul A Waters Elizabeth J |
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Affiliation: | The Australian Wine Research Institute, P.O. Box 197, Glen Osmond (Adelaide), SA 5064, Australia. |
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Abstract: | Bentonite is commonly used to remove grape proteins responsible for haze formation in white wines. Proteases potentially represent an alternative to bentonite, but so far none has shown satisfactory activity under winemaking conditions. A promising candidate is AGP, a mixture of Aspergillopepsins I and II.; a food grade, well characterized and inexpensive protease, active at wine pH and at high temperatures (60-80°C). AGP was added to two clarified grape juices with and without heat treatments (75°C, 1min) prior to fermentation. AGP showed some activity at fermentation temperatures (≈20% total protein reduction compared to control wine) and excellent activity when combined with juice heating (≈90% total protein reduction). The more heat stable grape proteins, i.e. those not contributing to wine hazing, were not affected by the treatments and therefore accounted for the remaining 10% of protein still in solution after the treatments. The main physicochemical parameters and sensorial characteristics of wines produced with AGP were not different from controls. |
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Keywords: | AGP Aspergillopepsin I and II PR-proteins pathogenesis-related proteins TLPs thaumatin-like proteins LTPs lipid transfer proteins MW molecular weight standards NTU nephelometry turbidity units Inv vacuolar invertases β-gluc β-1 3-glucanase Chit chitinases Barwin Barley PR-protein |
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