测定吡虫啉农药制剂中有效成分的3种HPLC不同定量方法比较

采用高效液相色谱法测定吡虫啉农药制剂产品中有效成分含量,并对单点校正法、标准曲线法和标准加入法3种不同方法进行分析,比较了3种方法的差异性。利用Excel对3种定量方法的标准偏差、基质效应和测定结果进行F检验和t检验。结果表明,在显著性水平α=0.05时,单点校正法与标准曲线法、标准加入法标准偏差有明显差别,标准曲线法和标准加入法之间无显著性差异。对可湿性粉剂产品,基质效应无法忽略,3种方法测定结果间均具有显著性差别;对乳油产品,标准曲线法和标准加入法测定结果无明显差别,但单点校正法与其他2种方法具有显著性差异。建议对可湿性粉剂产品选择标准加入法定量,乳油产品选择标准曲线法定量。

Comparation of Three Quantitative Methods for Determination of Imidacloprid by HPLC

The content of imidacloprid in pesticide product was determined by high performance liquid chromatography(HPLC),and quantitatively analysed by single point calibration method,standard curve calibration method and standard addition method.The significant differences of three methods were compared.The standard deviation,matrix effect and measurement results of the three quantitative methods were analyzed by using Excel for F-test and t-test statistical analysis.Results showed that the level of significance α=0.05,the significant differences of standard deviation were between signal point calibration method and other two methods,and there were no significant differences between standard curve calibration method and standard addition method.For wettable powder products,the matrix effect could not be ignored that there were significant differences among the results of the three methods.There was no significant difference in the measurement results of emulsifiable concentrate between the standard curve method and the standard addition method,but the single point correction method was significantly different from the other two methods.It is suggested to determine imidacloprid in wettable powder by standard addition method and in emulsifiable concentrate by standard curve calibration method.