| 基于肝微粒体和肝S9成分的食品接触材料中 五氯苯酚体外代谢分析 |
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| 引用本文： | 龚强,丁利,朱绍华,成婧,焦艳娜,付善良,王利兵.基于肝微粒体和肝S9成分的食品接触材料中 五氯苯酚体外代谢分析[J].食品安全质量检测技术,2016,7(1):108-112. |
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| 作者姓名： | 龚强 丁利 朱绍华 成婧 焦艳娜 付善良 王利兵 |
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| 作者单位： | 湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心,湖南出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 国家科技支撑计划项目(2012BAD29B05) |
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| 摘 要： | 目的通过体外代谢方法,研究五氯苯酚(pentachlorophenol,PCP)的代谢情况。方法采用肝微粒体和肝S9成分2种体外代谢试剂模拟体内代谢模式,通过优化代谢反应条件,建立PCP、四氯苯醌(Cl4BQ)2种目标物的气相色谱-质谱联用(GC-MS)检测方法,对PCP进行体外代谢研究分析。结果体外代谢分析的最佳反应条件为代谢试剂浓度0.75 mg/m L、代谢反应时间4 h。结论在最优反应条件下分析比较2种试剂对PCP的代谢情况,验证了PCP的代谢产物为Cl4BQ,且在肝微粒体和肝S9成分中的代谢没有显著差异。
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| 关 键 词： | 五氯苯酚 四氯苯醌 肝微粒体 肝S9成分 GC-MS 体外代谢 |
| 收稿时间： | 2015/12/1 0:00:00 |
| 修稿时间： | 2015/12/31 0:00:00 |
Metabolic study of pentachlorophenol in food contact materials by liver mi-crosome and liver S9 fraction |
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| GONG Qiang,DING Li,ZHU Shao-Hu,CHENG Jing,JIAO Yan-N,FU Shan-Liang and WANG Li-Bing.Metabolic study of pentachlorophenol in food contact materials by liver mi-crosome and liver S9 fraction[J].Food Safety and Quality Detection Technology,2016,7(1):108-112. |
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| Authors: | GONG Qiang DING Li ZHU Shao-Hu CHENG Jing JIAO Yan-N FU Shan-Liang and WANG Li-Bing |
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| Affiliation: | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau |
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| Abstract: | Pentachlorophenol (PCP) is a kind of very important preservative, mostly used in textile and leather, to inhibit the growth of bacterial and fungi. It is a possible human carcinogen detected widely in the environment. A possible quinine metabolite of PCP may be tetrachloro-1, 4-benzoquinone (Cl4BQ). We mimiced the environment of the liver by two kinds of reagents: liver microsome and liver S9 fraction to study the metabolism of PCP. Metabolic results showed that Cl4BQ were produced in both of the reagent. The best metabolic reagent concentration is 0.75mg/mL, and the most suitable reaction time is 4 hours. After compare of the metabolic reaction of liver microsome and liver S9 fraction, we found there was no statistic difference between the two kinds of reagents. |
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| Keywords: | Pentachlorophenol tetrachloro-1 4-benzoquinone GC-MS Liver microsome Liver S9 fraction metabolism in vitro |
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