| 食品包装材料中丙烯酰胺在大鼠肝微粒和S9中的体外代谢研究 |
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| 引用本文： | 付善良,丁 利,朱绍华,龚强,焦艳娜,王利兵.食品包装材料中丙烯酰胺在大鼠肝微粒和S9中的体外代谢研究[J].食品安全质量检测技术,2016,7(1):97-101. |
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| 作者姓名： | 付善良 丁 利 朱绍华 龚强 焦艳娜 王利兵 |
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| 作者单位： | 湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室,湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室,湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室,湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室,湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室,湖南出入境检验检疫局技术中心 食品安全科学技术湖南省重点实验室 |
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| 基金项目： | 国家科技支撑计划(2012BAD29B05) |
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| 摘 要： | 目的研究食品包装材料中丙烯酰胺(acrylamide)的体外代谢情况,并确证其代谢产物。方法采用肝微粒体及肝S9体外温孵法,优化代谢条件,对丙烯酰胺进行了体外代谢研究,并用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测并确证丙烯酰胺的代谢物。结果通过检测结果发现,丙烯酰胺分别在肝S9和肝微粒体作用下发生显著的代谢反应,其代谢产物之一为环氧丙酰胺。结论丙烯酰胺能通过代谢转化成毒性更强的环氧丙酰胺,因此,监控食品、饮水和周围环境中的丙烯酰胺的含量对于维护人们的健康具有重要的意义。
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| 关 键 词： | 食品包装 丙烯酰胺 代谢 肝微粒 S9 |
| 收稿时间： | 2015/12/1 0:00:00 |
| 修稿时间： | 2016/1/11 0:00:00 |
Metabolism of acrylamide by rat liver microsomes and S9 in vitro |
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| FU Shan-Liang,DING Li,ZHU Shao-Hu,GONG Qiang,JIAO Yan-Na and WANG Li-Bing.Metabolism of acrylamide by rat liver microsomes and S9 in vitro[J].Food Safety and Quality Detection Technology,2016,7(1):97-101. |
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| Authors: | FU Shan-Liang DING Li ZHU Shao-Hu GONG Qiang JIAO Yan-Na and WANG Li-Bing |
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| Affiliation: | Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology,Technology Center of Hunan Entry-Exit Inspection and Quarantine Bureau,Hunan Key Laboratory of Food Safety Science Technology |
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| Abstract: | Objective To investigate the metabolism and the metabolin of acrylamide in rat liver microsomes and S9 in vitro. Methods The metabolism was researched in rat liver microsomal and S9 incubation systems. A LC-MS/MS method was developed to determine acrylamide and its metabolin. Results Acrylamide was evidently metabolized by rat liver microsomes and S9, and one of the metabolins was found to be glycidamide. Conclusion Acrylamide can be metabolized to more toxic compound, glycidamide. Therefore, it is of great significance to monitor acrylamide in food, drinking water and the surrounding environment, for the maintenance of people's health. |
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| Keywords: | acrylamide metabolism liver microsomes |
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