| 铅的极谱络合吸附波法检测DNA特定序列 |
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| 引用本文： | 张波,刘鹤,焦奎,付洵,孙伟.铅的极谱络合吸附波法检测DNA特定序列[J].青岛科技大学学报,2009,30(2). |
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| 作者姓名： | 张波 刘鹤 焦奎 付洵 孙伟 |
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| 作者单位： | 青岛科技大学,化学与分子工程学院,山东,青岛,266042
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| 基金项目： | 国家自然科学基金，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 应用极谱络合吸附波方法检测了草丁膦乙酰转移酶基因(PAT基因)的DNA特定序列。用水热法合成表面具有自由羧基的硒化铅(PbSe)纳米粒子,以乙基-(3-二甲基丙基)碳二亚胺盐酸盐(EDC)为偶联活化剂,将其标记于合成的5′端氨基修饰的寡聚核苷酸片段上,制成DNA探针,用于检测互补的目标DNA序列。以十六烷基三甲基溴化铵(CTAB)阳离子表面活性剂修饰的碳糊电极作为基底电极,通过静电作用将来自于PAT基因的目标DNA特定序列固定在电极表面,并与标记有PbSe纳米粒子的互补DNA探针杂交。以铅-铜铁试剂-六次甲基四胺-HAc极谱络合吸附波测定氧化溶解Pb-Se得到的Pb2+,成功检测了PAT基因的DNA特定序列。测定目标DNA的线性范围为1.0×10-11至1.0×10-7mol.L-1,检测限为8.7×10-12mol.L-1(3σ)。此方法测定目标DNA序列检测限低,灵敏度高,线性范围宽,重现性好,操作简捷方便,对互补和非互补序列具有很好的识别能力。
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| 关 键 词： | DNA检测 极谱络合吸附波 硒化铅纳米粒子 DNA杂交 PAT基因 |
Detection for DNA Specific Sequences Using the Polarographic Complex Adsorptive Wave of Pb |
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| ZHANG Bo,LIU He,JIAO Kui,FU Xun,SUN Wei.Detection for DNA Specific Sequences Using the Polarographic Complex Adsorptive Wave of Pb[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2009,30(2). |
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| Authors: | ZHANG Bo LIU He JIAO Kui FU Xun SUN Wei |
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| Affiliation: | College of Chemistry and Molecular Engineering;Qingdao University of Science and Technology;Qingdao 266042;China |
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| Abstract: | The polarographic complex adsorptive wave was firstly used to detect DNA specific sequences of the phosphinothricin acetyltransferase(PAT)gene.Lead selenide(PbSe) nanoparticle surface-modified by free carboxyl was hydrothermally synthesized,and used as a marker to label the DNA probe for identifying complementary target DNA sequence.Sequence-specific DNA related to the PAT gene was used as target DNA sequence and immobilized electrostatically on cetyltrimethylammonium bromide(CTAB) modified carbon paste ele... |
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| Keywords: | DNA detection polarographic complex adsorptive wave PbSe nanoparticle DNA hybridization PAT gene |
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