| B.t.蛋白的纯化与鉴定 |
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| 引用本文： | 孙锋,林毅,张宽朝.B.t.蛋白的纯化与鉴定[J].农药,2006,45(12):825-827. |
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| 作者姓名： | 孙锋 林毅 张宽朝 |
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| 作者单位： | 安徽农业大学,生命科学学院,合肥,230036 |
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| 摘 要： | 通过碱溶、碱解的方法溶解B．t．蛋向，采用60％饱和度的硫酸钱粗提，Sephadex G-50层析凝胶分离，加1．5倍的95％乙醇可得晶体沉淀计冻干，所得B．t．蛋白含量为92％，SDS-PAGE电泳图谱显示所纯化的B．t．蛋白分子量为130KD，生物学检测时，幼虫至3铃虫的死亡卒均大于90％，生物活性达18000IU／mg，可T作抗原用。
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| 关 键 词： | B.t.蛋白 纯化 鉴定 |
| 文章编号： | 1006-0413（2006）12-0825-03 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-02-212006-04-09 |
Purification and identification of B.t. protein |
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| SUN Feng,LIN Yi,ZHANG Kuan-chao.Purification and identification of B.t. protein[J].Pesticides,2006,45(12):825-827. |
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| Authors: | SUN Feng LIN Yi ZHANG Kuan-chao |
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| Affiliation: | School of Life Science, Anhui Agriculture University, Hefei 230036, China |
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| Abstract: | B.t. protein was dissolved in alkaline solution, and solid ammonium sulphate was added into the above solution till 60% saturation. The precipitate was separated by Sephadex G-50, washed with 1.5 times of 95% ethanol, and freeze-dried. The content of B.t. toxin was 92%. The SDS-PSGE showed that the molecular of the B.t.protein was 103KD. The death rate of the bollworm with larva to second instar larva was more than 90%, and biological activity was 18 000IU/mg. The B.t. toxin could be uesed as antigen. |
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| Keywords: | B t protein purification identification |
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