Optimization of the Production of Total Carotenoids by Sporidiobolus salmonicolor (CBS 2636) Using Response Surface Technique |
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Authors: | Eunice Valduga Alexsandra Valério Helen Treichel Agenor Furigo Júnior Marco Di Luccio |
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Affiliation: | 1. Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, Rio Grande do Sul, 99700-000, Brazil 2. Department of Chemical and Food Engineering, UFSC, Florianópolis, Santa Catarina, 88040-900, Brazil
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Abstract: | This work aimed at evaluating the conditions of growth and the recovery of total carotenoids produced by Sporidiobolus salmonicolor (CBS 2636). Optimization of carotenoid production was achieved by experimental design technique. A Plackett–Burman design was used, followed by a complete second-order design, to optimize the concentration of total carotenoids in a conventional medium. Maximum concentration of 1,019 μg l−1 of total carotenoids was obtained in a medium containing 40 g l−1 glucose, 10 g l−1 malt extract, and 14 g l−1 peptone, at 180 rpm, 25 °C, and initial pH of 4.0. So far, no previous systematic study using microorganisms of the genus Sporobolomyces (formerly Sporidiobolus) for production of carotenoids has been reported. In this study, very good yields of carotenoids (1 mg l−1) could be obtained after optimization of fermentation medium and operation conditions. |
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