| 菲律宾蛤仔氨基多糖的分离提取及其抗肿瘤活性的初步研究 |
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| 引用本文： | 范秀萍,吴红棉,雷晓凌,钟佩茹,骆志华,谢莹.菲律宾蛤仔氨基多糖的分离提取及其抗肿瘤活性的初步研究[J].食品工业科技,2003,24(9):73-76. |
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| 作者姓名： | 范秀萍 吴红棉 雷晓凌 钟佩茹 骆志华 谢莹 |
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| 作者单位： | 湛江海洋大学水产学院,湛江,524025 |
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| 摘 要： | 以湛江地区盛产的菲律宾蛤仔全脏器为原料,经酶解、脱色、离心去蛋白后醇沉、干燥等工序得到氨基多糖粗制品(CRG),再将所得的CRG经一次纯化(吸附、透析)、二次纯化(CTAB络合)、醇沉等方法进行纯化,得到菲律宾蛤仔纯化产物(PRG)。PRG含有氨基已糖、乙糖醛酸、硫酸基和少量的岩藻糖(Fuc)、半乳糖(Gal),琼脂糖凝胶电 泳可分为F—1、F—2两个级分。红外光谱与CS—6相似。体外抗肿瘤实验表明,1.0mg/mL菲律宾蛤仔CRG具有显著的抗肿瘤活性,24h内对HL—60细胞的抑制率达59.8％。
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| 关 键 词： | 菲律宾蛤仔 氨基多糖 分离提取 抗肿瘤活性 |
| 文章编号： | 1002-0306(2003)09-0073-04 |
| 修稿时间： | 2003年1月20日 |
Isolation and extraction of glycosaminoglycan from Ruditapes philippinarum and the research on its anti-tumor activity |
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| Fan Xiuping et al.Isolation and extraction of glycosaminoglycan from Ruditapes philippinarum and the research on its anti-tumor activity[J].Science and Technology of Food Industry,2003,24(9):73-76. |
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| Authors: | Fan Xiuping |
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| Affiliation: | Fan Xiuping et al |
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| Abstract: | The researches were carried out on the isolation and extraction of glycosaminoglycan(GAG) and its
anti -tumor activity from Ruditapes philippinarum,
which is abundant in the sea areas around Zhenjiang city. The crude product of GAG(CRG) was isolated by enzyme hydrolysis and precipitation with alcohol. The purified product of GAG(PRG) was separated with alcohol and CTAB successively.The PRG contained hexosamine, hexosuronic acid.sulfate and fucose and galactose. The results of agarose gel electrophoresis showed that the PRG can be divided into two fractions (F-1,F-2).The graphs of infrared spectrometry were similar to CS-6's. The 1.0mg/mL CRG had the remarkable activity in killing the HL-60 cells, its killing rate was 59.8% in 24h. |
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| Keywords: | Ruditapes philippinarum glycosaminoglycan isolation and extraction anti-tumor activity |
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