微波消解-氢化发生原子荧光光谱法测定水产食品中的微量砷

利用微波消解-氢化发生原子荧光光谱法测定水产食品中微量砷的含量,实验考察了对测量影响较大的仪器工作参数(如灯电流、负高压等)和氢化发生条件(如载气流速、酸介质、硼氢化钾浓度、预还原剂等)。在优化实验条件下测定了几种水产食品中微量砷的含量及紫菜中无机砷的测定,平均加标回收率为96.3%,具有较高的测量稳定性(RSD<5.9%),样品空白测定方法的检出限(3σ)为0.021μg/kg。结果表明方法具有较高的测量灵敏度,稳定可靠,可应用于水产食品中微量及痕量砷及砷形态的检测。

Determination of Trace Arsenic in Aquatic Foods by Hydride Generation-atomic Fluorescence Spectrometry

The trace arsenic in aquatic foods was measured by microwave digestion coupled with the hydride generation-atomic fluorescence spectrometry (HG-AFS). The instrumental parameters and analytical conditions, including lamp (As-HCl) current, carrier gas flow, atomization stove temperature, acid medium, KBH4 concentrations and reducing agents, were investigated and optimized. Under optimum conditions linear response ranged from 0 to 80μg/Lwith R2≥0.999. The average recovery tested with adding standards was 96.3%, and the detection limit estimated with 3-fold standard deviation was 0.021μg/kg. The established method, with the advantages of simple operation, high sensitivity, and high efficiency, was successfully used for the determination of As in some aquatic foods.