薄层扫描法测定罗汉果糖苷(V)含量的研究

建立采用薄层扫描法测定罗汉果糖苷(V)含量的方法。点样于硅胶G板上,以正丁醇-乙醇-水(8∶2∶3)为展开剂,10 %硫酸乙醇显色,单波长反射法锯齿扫描,λ=5 0 0 nm。罗汉果糖苷(V)在2 .0～16 . 0 μg范围内具有良好的线性关系,r=0 .992 1,平均回收率为97.6 2 % ,RSD为2 .5 9% (n=4 )。该法简便快速、准确灵敏,可用于罗汉果糖苷生产质量监控。

Determination of mogroside V by thin layer scan

To develop a thin layer scanning method for the determination of mogroside V.Development on G silica gel board with n-butanol-ethanol-water(8∶2∶3) as developing solvent,coloration with an alcoholic solution of sulfuric acid (10%) and determination of densitometry by single wavelength-Zig Zag-reflection scan made at λ=500nm were adopted.The calibration curves were linear between2.0～16.0μg (r=0.9921).The average recovery was 97.62%.RSD was 2.59%(n=4).This method is simple ,rapid and accurate ,and can be used for the quality control in the production of mogroside.