单链DNA在氨基乙硫醇单分子膜金电极上固定化的研究

利用了氨基乙硫醇在金电极表面形成自组装单分子膜，然后用水溶性的碳化二亚胺作为偶联活化剂，ｓＤＮＡ的５’端磷酸基与电极表面自组装膜上的氨基以磷酸氨基酯键的形式共价结合，从而在金电极表面形成ｓｓＤＮＡ单分子层。采用反射红外光谱对电极表面ｓＤＮＡ单分子层进行了表征，并用氧化还原电对Ｃｏ（ｂｐｙ）３＋／２＋３作为杂交指示剂对电极表面的ｓＤＮＡ层的响应特性进行了研究。

Study on Immobilization of ssDNA onto Au Electrode Coating Aminoethanethiol Monolayer

In this paper, Self assembled technique was utilized to immobilize ssDNA on Au electrode. An active self assembled monolayer was prepared by coating aminoethanethiol on Au electrode surface, the 5' terminal phosphated end of ssDNA formed a phosphormidate bond with the amino group of aminoethanethiol on the Au electrode. The characteristics of IR spectra of ssDNA monolayers on the electrode surface were studied. The covalent immobilization of DNA using the redox couple Co(bpy) 3 3 as indictor for the surface quanlity of ssDNA on the electrode was also investigated.