蒸汽萃取-高效液相色谱紫外/荧光法同时测定果蔬中噻苯咪唑、邻苯基苯酚、联苯及抑霉唑残留

建立了一种快速同时测定果蔬中噻苯咪唑（TBZ）、邻苯基苯酚（OPP）、联苯（DP）及抑霉唑（IMZ）残留量的高效液相色谱紫外/荧光分析方法。柑橘类水果及蔬菜样品经组织捣碎机打碎研磨均匀后,利用咖啡萃取机产生的甲醇或乙醇蒸汽进行萃取,分别采用紫外/荧光检测器同时测定上述四种或三种防腐保鲜剂。结果表明：紫外检测TBZ、OP...

Steam Extraction and Simultaneous Determination of Thiabendazole,O-phenyl phenol,Diphenyl and Imazalil Residues in Citrus Fruits and Vegetables by HPLC with Ultraviolet/Fluorescence Detection

A rapid reversed-phase HPLC method is developed for simultaneous determination of thiabendazole（TBZ）,o-phenylphenol（OPP）,dipheny（DP） and imazalil（IMZ） in citrus fruits and vegetables.The samples,blendering and pulverizing,are extracted by methanol or ethanol steam,producing by coffee machine;and subsequently assay above four or three preservatives by HPLC with ultraviolet/fluorescence detection,respectively.The study shows that TBZ、OPP、IMZ、DP have good linear response within concentration range（0.5～10）μg/ml,（0.6～10）μg/ml,（1～80）μg/ml,（0.8～20）μg/ml,lowest detection leves are 0.4,0.4,0.5,0.8μg/ml respectively,determining by ultraviolet detection;TBZ、OPP、DP,determining by ultraviolet detection,linear ranges are（0.05～2.0）μg/ml,（0.06～2.0）μg/ml,（0.08～5.0）μg/ml,lowest detection leves are 0.02,0.03,0.05g/ml.Using methanol and ethanol steam,ether solvent as extraction mediums,obtaining recoveries of spiked samples ranged from 67%～84%,72%～83%,52%～108% and relative standard deviations are from 1.23%～3.57%,0.35%～2.35%,1.71%～6.29%.The method is simple,fast and accurate,meeting the demands for simultaneously detecting preservatives multiresidues in fresh fruits and vegetables.