Biosynthesis of hydrocarbons by algae: Decarboxylation of stearic acid to N-heptadecane inAnacystis nidulans determined by13C- and2H-labeling and13C nuclear magnetic resonance |
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Authors: | A Gavin McInnes John A Walter Jeffrey L C Wright |
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Affiliation: | (1) Atlantic Research Laboratory, National Research Council of Canada, 1411 Oxford Street, B3H 3Z1 Halifax, Nova Scotia, Canada |
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Abstract: | The distribution of isotopic labels inn-heptadecane enriched from 1,2-13C] and 2-13C, 2-2H3) acetates byAnacystis nidulans has been determined by13C nuclear magnetic resonance (13C NMR). Labeling with 1,2-13C] acetate is consistent with assembly from13C−13C units derived from an acetate “starter” group and 8 malonate units, as in fatty acid biosynthesis, followed by production
of a methyl group through bond cleavage of the terminal13C−13C unit. A comparison of the hydrocarbon with palmitic acid (the only fatty acid produced in sufficient amount for NMR analysis)
enriched from 2-13C,2-2H3]acetate by the same culture shows that they have retained the same fraction of2H at corresponding sites, and have therefore undergone identical biosynthetic and hydrogen-deuterium exchange processes, as
would be expected ifn-heptadecane originates from de novo-synthesized stearic acid.
NRCC No. 18251. |
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