Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 6: 3D hydrogels with positive and negative surface charges and polyelectrolyte complexes in spinal cord injury repair |
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Authors: | A Hej?l P Lesný M P?ádný J ?edý J Záme?ník P Jendelová J Michálek E Syková |
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Affiliation: | A. Hej?l, P. Lesny, M. P?ádny, J. ?edy, J. Záme?ník, P. Jendelová, J. Michálek and E. Syková |
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Abstract: | Macroporous hydrogels are artificial biomaterials commonly used in tissue engineering, including central nervous system (CNS)
repair. Their physical properties may be modified to improve their adhesion properties and promote tissue regeneration. We
implanted four types of hydrogels based on 2-hydroxyethyl methacrylate (HEMA) with different surface charges inside a spinal
cord hemisection cavity at the Th8 level in rats. The spinal cords were processed 1 and 6 months after implantation and histologically
evaluated. Connective tissue deposition was most abundant in the hydrogels with positively-charged functional groups. Axonal
regeneration was promoted in hydrogels carrying charged functional groups; hydrogels with positively charged functional groups
showed increased axonal ingrowth into the central parts of the implant. Few astrocytes grew into the hydrogels. Our study
shows that HEMA-based hydrogels carrying charged functional groups improve axonal ingrowth inside the implants compared to
implants without any charge. Further, positively charged functional groups promote connective tissue infiltration and extended
axonal regeneration inside a hydrogel bridge. |
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