| 石榴皮原花青素的纯化工艺及其抗李斯特菌活性研究 |
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| 引用本文： | 柯春林,孙兰萍,张斌,曾卫国,吕超田,程立胜. 石榴皮原花青素的纯化工艺及其抗李斯特菌活性研究[J]. 陕西化工, 2014, 0(2): 261-264 |
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| 作者姓名： | 柯春林 孙兰萍 张斌 曾卫国 吕超田 程立胜 |
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| 作者单位： | 蚌埠学院生物与食品工程系,安徽蚌埠233030 |
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| 基金项目： | 安徽省高等学校省级自然科学基金项目（KJ2012B094）;蚌埠学院博士科研启动基金项目;蚌埠学院优秀人才基金项目（院字[2009]107号） |
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| 摘 要： | 从6种大孔树脂中筛选出对石榴皮原花青素纯化效果较好的HPD-600树脂,探索该树脂对原花青素的纯化工艺.结果表明,该树脂纯化原花青素的最佳条件是：静态平衡时间8h,提取液pH值为5.0,吸附液料比为12∶1,静置吸附时间90 min,洗脱液为80％的乙醇溶液,洗脱液料比25∶1.原花青素提取液对单增李斯特菌有抑菌作用.
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| 关 键 词： | 石榴皮 原花青素 纯化工艺 李斯特菌 抗菌活性 |
Research for the purification conditions of proanthocyanidins from pomegranate peels and its antibacterial activity to Listeria monocytogenes |
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| KE Chun-lin,SUN Lan-ping,ZHANG Bin,ZENG Wei-guo,LU Chao-tian,CHENG Li-sheng. Research for the purification conditions of proanthocyanidins from pomegranate peels and its antibacterial activity to Listeria monocytogenes[J]. Applied Chemical Industry, 2014, 0(2): 261-264 |
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| Authors: | KE Chun-lin SUN Lan-ping ZHANG Bin ZENG Wei-guo LU Chao-tian CHENG Li-sheng |
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| Affiliation: | ( Department of Biotechnology and Food Engineering, Bengbu College, Bengbu 233030, China) |
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| Abstract: | In order to purified the proanthocyanidins from pomegranate peels, selected six kinds of macroporous resins in this experiment and found that the HPD-600 macroporous resin showed more better separation performance to the proanthocyanidins. Result showed that the optimum conditions were as follows： static equilibrium time 8 h, extract pH value 5.0,adsorption liquid to solid ratio 12：1 ,static adsorption time 90 rain, eluting with 80% the ethanol solution, eluent liquid to solid ratio 25 ： 1. The proanthocyanidins from pomegranate peels showed moderate antibacterial activity to the Listeria monocytogenes. |
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| Keywords: | pomegranate peels proanthocyanidins purification conditions Listeria monocytogenes antibacterial activity |
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