| 非标记适体-金纳米检测尿中痕量腺苷 |
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| 引用本文： | 赵慧,王爱芝,王永生.非标记适体-金纳米检测尿中痕量腺苷[J].陕西化工,2014(3):562-564. |
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| 作者姓名： | 赵慧 王爱芝 王永生 |
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| 作者单位： | 南华大学公共卫生学院,湖南衡阳421001 |
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| 基金项目： | 湖南省科技厅科技计划资助项目（2010SK3039） |
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| 摘 要： | 在pH 4.6 BR缓冲溶液中,腺苷与腺苷适体特异性结合,金纳米不被腺苷适体保护而在高盐浓度下聚集,导致共振光散射强度增强,且在1.9×10-12～2.0 ×10-11 mol/L范围内,体系共振光散射强度的增强值（ΔI）与腺苷浓度呈良好线性关系,其回归方程为ΔI=71.3＋17.0c（×10-12mol/L）,r=0.994 8,检出限为5.7×10-13 mol/L,相对标准偏差（RSD）为1.48％～ 3.70％.用于尿样中腺苷的测定,回收率在97.1％～102.8％.该方法简单、快速、灵敏.
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| 关 键 词： | 腺苷 金纳米 适体 共振光散射 |
Determination of trace adenosine in human urine using unlabelled aptamer-AuNPs |
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| ZHAO HuiCollege of Public Health,University of South China,Hengyang,China WANG Ai-zhiCollege of Public Health,University of South China,Hengyang,China WANG Yong-sheng.Determination of trace adenosine in human urine using unlabelled aptamer-AuNPs[J].Applied Chemical Industry,2014(3):562-564. |
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| Authors: | ZHAO HuiCollege of Public Health University of South China Hengyang China WANG Ai-zhiCollege of Public Health University of South China Hengyang China WANG Yong-sheng |
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| Affiliation: | ZHAO Hui(College of Public Health, University of South China, Hengyang 421001, China) WANG Ai-zhi(College of Public Health, University of South China, Hengyang 421001, China) WANG Yong-sheng(College of Public Health, University of South China, Hengyang 421001, China) |
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| Abstract: | In the Britton-Robinson （BR） buffer solution of pH 4.6,adenosine could combine with aptamer specially.Gold nanoparticles （AuNPs） was not protected by aptamer and aggregated under high salt concentration.This resulted in the enhancement of resonance light scattering （RLS） intensity.ΔI Was directly proportional to the concentration of adenosine in the range of 1.9 × 10-12 ～ 2.0 × 10-11 mol/L.The equation of linear regression was ΔI =71.3 ＋ 17.0 c （× 10-12 tmol/L）,r =0.994 8.The limit of detection （LOD） was 5.7 ×10-13 mol/L.The relative standard deviations were 1.48％ ～3.70％,and the recovery was 97.1％ ～ 102.8％.The proposed method was simple,rapid and sensitive. |
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| Keywords: | adenosine gold nanoparticles aptamer resonance light scattering |
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