用Cytb基因序列分析鉴定肉制品掺假

目的采用Cytb基因进行肉制品真伪鉴定的尝试性探索。方法随机采集21份肉制品样品,首先采用CTAB法方法提取样品DNA,基于Cytb基因的通用引物对21份样品DNA进行PCR扩增,把扩增产物进行测序,然后采用DNAMAN软件进行序列的比对分析。结果 CTAB方法适合肉制品的DNA提取。10份牛肉样品中9份与标注吻合,1份检测出是猪肉;2份羊肉样品中1份与标注吻合,1份检测是猪肉;2份猪肉样品中1份与标注吻合,1份检测是鸡肉;1份鸡肉样品与标注吻合;6份牦牛肉样品中2份与标注吻合,4份检测是水牛肉。结论用Cytb基因序列分析比对方法适合肉制品的真伪鉴定,市场上肉制品存在掺假问题。

Meat identification based on Cytb gene sequences for the market meat products

To explore the identification of the meat and meat products based on Cytb gene.Methods A total of 21 samples were collected randomly. DNAs were extracted by CTAB method. Conventional PCR technique was applied to amplify Cytb genes and the amplified PCR products were sequenced. DNAMAN software was used to align the Cytb sequences. Results CTAB method was suitable for DNA extraction in meat products. The DNA sequencing and alignment results indicated that 9 beef samples were consistent with the label, and one was pork; one mutton sample was consistent with label, another was detected as pork; one pork sample was consistent with label, and the other was detected as chicken; one chicken sample was consistent with label; 2 yak samples were consistent with label, and four were detected as water buffalo meats.Conclusion Sequences alignment method based on Cytb gene was suitable for the market meat products identification. Moreover, meat adulteration was very serious.