| 脂肪酶催化拆分环戊烯酮动力学研究 |
| |
| 引用本文： | 丁永学,张铭俊,虞星炬. 脂肪酶催化拆分环戊烯酮动力学研究[J]. 陶瓷科学与艺术, 1999, 0(3) |
| |
| 作者姓名： | 丁永学 张铭俊 虞星炬 |
| |
| 作者单位： | 中国科学院大连化学物理研究所生化工程研究室 |
| |
| 摘 要： | 考察了４种不同来源的脂肪酶对环戊烯酮的拆分活性;从中选择ＰｓｅｕｄｏｍｏｎａｓＣｅｐａｃｉａＬｉｐａｓｅ为实验用酶,考察了影响酶拆分反应动力学的几种因素,结果表明酶拆分的最适温度为４０℃,ｐＨ值为７．０;反应过程存在严重的底物抑制,反应最终得到质量分数为４６．５％单一旋光纯的（Ｒ）－环戊烯酮。
|
| 关 键 词： | 酶拆分 环戊烯酮 反应动力学 |
KINETICS OF ENZYME RESOLUTION OF = ()4HYDROXY3METHYL2(2PROPENYL)CYCLOPENTEN1ONE CATALYZED BY %LIPASE |
| |
| Ding Yongxue Zhang Mingjun Yu Xingju=. KINETICS OF ENZYME RESOLUTION OF = ()4HYDROXY3METHYL2(2PROPENYL)CYCLOPENTEN1ONE CATALYZED BY %LIPASE[J]. Ceramics Science & Art, 1999, 0(3) |
| |
| Authors: | Ding Yongxue Zhang Mingjun Yu Xingju= |
| |
| Abstract: | The enzyme resolution of ()4Hydroxy3Methyl2(2propenyl)Cyclopenten1one catalyzed by 4 {%Lipases%} from different sources was studied separately. The kinetics of the hydrolysis reaction catalysed by the specific lipase from Pseudomonas Cepacia sp were examined. It was found that there was strongly reactant inhibition. The {reactions} optimal temperature and pH were 40 and 7.0 respectively. In the experiment we finally prepared 46.5% (R)4Hydroxy3Methyl2(2proneyl)Cyclopenten1One with high optical purity.= |
| |
| Keywords: | enzyme resolution 4hydroxy3methyl2(2propenyl)cyclopenten1one kinetics |
| 本文献已被 CNKI 等数据库收录！ |
