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螺旋藻自身生长抑制物的去除及模型分析
引用本文:王利蒙,刘明,薛升长,丛威,卢运明. 螺旋藻自身生长抑制物的去除及模型分析[J]. 过程工程学报, 2012, 12(3): 478-483
作者姓名:王利蒙  刘明  薛升长  丛威  卢运明
作者单位:1. 中国科学院过程工程研究所生化工程国家重点实验室,北京100190;中国科学院研究生院,北京100049
2. 中国科学院过程工程研究所生化工程国家重点实验室,北京,100190
3. 深圳市绿得宝保健食品有限公司,广东深圳,518040
摘    要:使用不同吸附剂吸附长期培养螺旋藻的藻液,通过比较螺旋藻在处理后的藻液中的比生长速率,筛选出能去除胞外自身生长抑制物的吸附剂;比较吸附前后藻液中多糖、有机酸和蛋白含量,提取出差别最大的物质,测试其对螺旋藻生长的抑制效应,并用数学模型对吸附过程进行分析. 结果表明,螺旋藻自身生长抑制物为胞外多糖,用大孔吸附树脂S-8处理藻液可使多糖含量降低62%,比生长速率提高39.4%;S-8对藻液中多糖的吸附30 min即可达到平衡,假二级动力学模型和Boyd液膜扩散模型与动力学实验数据拟合较好,线性相关系数分别为0.9991和0.9374.

关 键 词:钝顶螺旋藻  藻液  自身生长抑制物  胞外多糖
收稿时间:2012-04-16
修稿时间:2012-05-17

Removal of Auto-growth Inhibitors of Spirulina platensis and Model Analysis
WANG Li-meng , LIU Ming , XUE Sheng-zhang , CONG Wei , LU Yun-ming. Removal of Auto-growth Inhibitors of Spirulina platensis and Model Analysis[J]. Chinese Journal of Process Engineering, 2012, 12(3): 478-483
Authors:WANG Li-meng    LIU Ming    XUE Sheng-zhang    CONG Wei    LU Yun-ming
Affiliation:1.National Key Lab.Biochem.Eng.,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Shenzhen Lvdebao Health Food Limited Company,Shenzhen,Guangdong 518040,China)
Abstract:A long-term used culture medium of Spirulina platensis was treated with different adsorbents,and they were screened in removal of the contained inhibitors via comparison of specific growth rate of Spiruina platensis in different adsorption-treated culture media.The concentrations of polysaccharide,organic acid and protein in culture medium before and after adsorption were compared,and the one with the largest concentration disparity was extracted to test its inhibition effect on Spirulina platensis growth.In addition,model analysis was carried out on the adsorption process.The results showed that the auto-growth inhibitor of Spirulina platensis was extracellular polysaccharide,and the adsorption treated culture medium by macro-porous adsorption resin S-8 could reduce extracellular polysaccharide content by 62% and simultaneously increased the specific growth rate by 39.4%,compared with the non-treatment medium.For the adsorption process of polysaccharide by S-8,30 min was sufficient for equilibrium,pseudo-second-order and Boyd film diffusion model fitted well with the kinetic data,with linear correlation coefficients of 0.9991 and 0.9374,respectively.
Keywords:Spirulina platensis  culture medium  auto-growth inhibitor  extracellular polysaccharide
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