Conjugated linoleic acid content of milk from buffaloes fed a mustard oil-based diet

Fifteen Murrah buffaloes were distributed in groups I, II and III. The group I animals were fed with groundnut cake-based concentrate, group II animals with mustard cake-based concentrate and group III with 2% of mustard oil added to the group II feed. Conjugated linoleic acid (CLA) estimation in milk was done by using GC. The average total CLA contents (mg/g milk fat) in the three groups were 6.84, 12.12 and 19.50 mg/g of fat respectively. Hence it was concluded that addition of 2% mustard oil resulted in a 185% increase in milk fat total CLA content.