| 正交优化燕麦β-葡聚糖提取及其分子特性研究 |
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| 引用本文： | 潘妍,吴昊.正交优化燕麦β-葡聚糖提取及其分子特性研究[J].北京轻工业学院学报,2009(5):5-9. |
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| 作者姓名： | 潘妍 吴昊 |
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| 作者单位： | 北京工商大学北京市植物资源研究开发重点实验室,北京100048 |
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| 基金项目： | 北京市科技新星项目（2008B08）. |
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| 摘 要： | 为确定提取燕麦中β-葡聚糖的最佳工艺条件，以分光光度法测定得到的燕麦β-葡聚糖含量为指标，采用单因素实验和正交试验设计，分别考察pH值、液料比、温度和提取时间对提取率的影响．由此知提取燕麦中的β-葡聚糖的较佳工艺是，液料比为25，pH值为11时80℃水浴提取4h．用凝胶色谱测定纯化后β-葡聚糖平均分子量，得其平均分子量为9．697×10^5D．
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| 关 键 词： | 燕麦 提取分离工艺 分子量 |
OPTIMIZATION OF OAT β-GLUCAN EXTRACTION TECHNOLOGY AND DETERMINATION OF MOLECULAR CHARACTERISTIC BY USING GPC |
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| PAN Yan,WU Hao.OPTIMIZATION OF OAT β-GLUCAN EXTRACTION TECHNOLOGY AND DETERMINATION OF MOLECULAR CHARACTERISTIC BY USING GPC[J].Journal of Beijing Institute of Light Industry,2009(5):5-9. |
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| Authors: | PAN Yan WU Hao |
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| Affiliation: | ( Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100048, China ) |
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| Abstract: | The extraction condition for β-glucans from oat bran was optimized. UV-spectrophotometry was used for the determination of β-glucans. The effects of β-glucans extraction were analyzed in several single factor experiments and orthogonal experiment. The results showed that the optimum conditions as follows： extraction temperature is 80℃, ratio of liquid to material is 25, pH is 11, and extract time is 4 h. The molecular weight of β-glucans after purified was obtained using high performance gel permeation chromatography （GPC）, and the average molecular weight is 9. 697×10^5D. |
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| Keywords: | oat extracting technology molecular weight |
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