| 好氧反硝化菌的物理诱变法育种研究 |
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| 引用本文： | 李永智,汪苹,王磊.好氧反硝化菌的物理诱变法育种研究[J].北京轻工业学院学报,2009(3):5-8. |
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| 作者姓名： | 李永智 汪苹 王磊 |
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| 作者单位： | 北京工商大学化学与环境工程学院,北京100048 |
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| 基金项目： | 国家“十一五”科技支撑计划项目（2007BAK36807）. |
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| 摘 要： | 以实验室SBR反应器分离出的好氧反硝化菌（WXZ-9）为原始菌株，分别用微波和紫外线诱变．通过反硝化性能测定筛选得到的正突变体WXZ-9—8号，96h后的TIN去除率达到67．20％，比出发菌株WXZ-9号菌提高了7．07％，NO3ˉ - N去除率在24h达到100％；WXZ-9—8号突变体的最大亚硝基氮还原速率达到52．89mgNO2ˉ-N·L^1·d^-1，比WXZ-9号菌株高6．90mgNO2ˉ—N·L^-1·d^-1．结果表明：WXZ-9—8号突变体具有更好的亚硝基氮还原酶的活性，并且比出发菌株有更好的生长适应性．
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| 关 键 词： | 好氧反硝化菌 微波诱变 紫外线诱变 |
STUDY ON MUTATION BREEDING WITH PHYSICAL METHODS BY AEROBIC DENITRIFIERS |
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| LI Yong-Zhi,WANG Ping,WANG Lei.STUDY ON MUTATION BREEDING WITH PHYSICAL METHODS BY AEROBIC DENITRIFIERS[J].Journal of Beijing Institute of Light Industry,2009(3):5-8. |
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| Authors: | LI Yong-Zhi WANG Ping WANG Lei |
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| Affiliation: | ( College of Chemical and Environmental Engineering, Beijing technology and Business University, Beijing 100048, China) |
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| Abstract: | Select aerobic denitrification strain（WXZ-9） isolated from SBR reactor in the laboratory as the original strain, mutate it with UV and microwave as the mutagens. The WXZ-9-8 Screening from mutants through mensured denitrification ability is the best, after 96 hours its reduction rate of TIN is 67.20 %, it＇s 7.07 % higher than the original strain, and the reduction rate of NO3-N reach 100 % ; The maximum diminishing speed of WXZ-9-8 reach 52.89 mgNO2ˉ-N·L^-1·d^-1, 6.90 mgNO2ˉ -N·L^-1·d^-1 higher than WXZ-9, so WXZ-9-8 has better activity of nitrite reductase, WXZ-9-8 has better adaptive growth too. |
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| Keywords: | aerobic denitrifiers microwave mutation UV-ray mutation |
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