莲原花青素对小鼠黑色素瘤的体内抑制作用及机制研究

目的:研究莲房原花青素(LSPC)对黑色素瘤B16的抑制作用及其机制。方法:给小鼠连续灌胃不同剂量的LSPC 13d后,于第14d处死小鼠,计算抑瘤率并进行形态学观察,通过免疫组织化学观察瘤细胞内HMB45和S-100蛋白表达,流式细胞术检测细胞凋亡。结果:一定剂量的LSPC能抑制黑色素瘤B16细胞生长增殖,且以120mg/kg·bw LSPC组最显著(p＜0．01),抑瘤率达55．3%,并有亚二倍凋亡峰。结论:LSPC对黑色素瘤B16细胞有抑制作用,其机制是诱导凋亡。

Inhibitory Effects of Proanthocyaidins from Lotus Seedpod (LSPC) on Mice B16 Melanoma

Objective:To investigate the inhibitory effects of proanthocyaidins from lotus seedpod（LSPC）on mice B16 melanoma.Methods:The mice were fed with different dosage LSPC continuely for 13d,on the 14thday,all mice were killed, and the inhibition rate of B16 melanoma was calculated.The changes of apoptosis cells in morphology were observed,and the expression of HMB45 and S-100 protein in B16 melanoma were tested by immune histochemistry method.Results:LSPC can inhibit the proliferation of B16 melanoma.The dosage of 120mg/kg·bw shows the most significantly difference in comparison with the control（p＜0.01）.The inhibition rate of B16 melanoma reaches 55.3%,and the cell apoptosis analysis presents a sub- peak.Conclusions:LSPC has the inhibitory effects on the B16 melanoma and the mechanism is to induce the apoptosis.