Characterization of cheese proteolysis by capillary electrophoresis and reverse-phase HPLC analyses of peptides |
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Authors: | Elena Molina Mercedes Ramos Alejandro Cifuentes and R López-Fandi?o |
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Affiliation: | (1) Instituto de Fermentaciones Industriales (C.S.I.C), Juan de la Cierva 3, E-28006 Madrid, Spain e-mail: ifilf34@ fresno.csic.es, ES;(2) Instituto de Química Orgánica (C.S.I.C.), Juan de la Cierva 3, E-28006 Madrid, Spain, ES |
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Abstract: | The possibilities of reverse-phase high performance liquid chromatography (RP-HPLC) and capillary electrophoresis (CE) when
used for separation of cheese peptides are discussed. A CE method using a coated capillary column and a low pH buffer was
developed to analyze the water-soluble fraction of a 6-month-old cow’s milk cheese. The CE patterns were compared with the
chromatograms obtained by RP-HPLC using a C18 column and a gradient of acetonitrile in water. The CE method gave shorter analysis
times but RP-HPLC provided lower coefficients of variation of the retention times and better detection limits. In addition,
the elution behavior of peptides in CE strongly depended on the sample matrix. The results show that both techniques provide
complementary information for the analysis of cheese peptides.
Received: 10 June 1997 / Revised version: 20 October 1997 |
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Keywords: | Capillary electrophoresis Reverse-phase high performance liquid chromatography Cheese peptides Water-soluble nitrogen |
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