斑玉蕈多糖的酶辅助提取及其抗氧化活性分析

通过Box-Behnken中心组合实验设计,获得了酶辅助提取斑玉蕈多糖的最佳工艺;以DPPH自由基清除率、还原力、羟基自由基清除率为指标,评价了斑玉蕈多糖的抗氧化活性。结果表明,酶辅助提取斑玉蕈多糖的最佳工艺条件为复合酶（木瓜蛋白酶和纤维素酶按1∶1质量比例配合）添加量0.9%、酶解pH5.4、酶解温度55.6℃、提取时间2.8h,在此条件下多糖得率为4.68%。斑玉蕈多糖具有较好的抗氧化活性,在一定范围内,其抗氧化能力与多糖质量浓度呈线性正相关,斑玉蕈多糖清除DPPH和羟基自由基的IC50值分别为78.5μg/mL和170.5μg/mL。 

Optimization of enzyme-assisted extraction and antioxidant activity of polysaccharides from Hypsizigus marmoreus

The optimum processing conditions of enzyme-assisted extraction of polysaccharides from Hypsizigus marmoreus were studied by Box-Behnken statistical design. The antioxidant activities of polysaccharides from Hypsizigus marmoreus were investigated by 1 , 1 - diphenyl - 2 - picrylhydrazyl ( DPPH ) radical scavenging activities, hydroxyl radical scavenging activities and reducing power. The results showed that when the extraction parameters were controlled at complex enzyme (i.e. papain and cellulase mixed at a mass ratio of 1∶1) amount 0.9% , enzymolysis pH5.4, enzymolysis temperature 55.6℃ , and extraction time 2.8h, the polysaccharides yield could be up to 4.68% . The positive liner correlations between the antioxidant activity and concentration of polysaccharides were found over the investigated ranges. IC 50 values against DPPH and hydroxyl radicals were 78.5μg/mL and 170.5μg/mL, respectively.