| 蝉拟青霉菌丝体糖蛋白的分离提取及组分测定 |
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| 引用本文： | 谭艾娟,梁宗琦,刘爱英. 蝉拟青霉菌丝体糖蛋白的分离提取及组分测定[J]. 食品科学, 2007, 28(1): 159-160 |
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| 作者姓名： | 谭艾娟 梁宗琦 刘爱英 |
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| 作者单位： | 贵州大学生命科学学院； 贵州大学生命科学学院 贵州贵阳550025； 贵州贵阳550025； |
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| 摘 要： | 蝉拟青霉菌粉经脱脂、水提、丙酮沉淀、蒸馏水透析后得到糖蛋白提取物GP,经苯酚-硫酸法测定总糖含量为34.79%;Lowry法测定蛋白质含量为63.45%;糖蛋白提取物GP上SephdexG-25柱层析,逐管用紫外吸收法(280nm)检测,发现有两个峰(PS1和PS2)。PS1总糖含量为21.11%,蛋白质含量为77.1%,PS2总糖含量为44.98%,蛋白质含量为53.02%。
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| 关 键 词： | 蝉拟青霉 菌丝体 总糖 糖蛋白 柱层析 |
| 文章编号： | 1002-6630（2007）01-0159-02 |
| 修稿时间： | 2006-06-08 |
Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test |
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| TAN Ai-Juan,LIANG Zong-Qi,LIU Ai-ying. Extraction and Separation of Glycoproteins from the Mycelia of Paecilomyces cicadae with Analysis Test[J]. Food Science, 2007, 28(1): 159-160 |
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| Authors: | TAN Ai-Juan LIANG Zong-Qi LIU Ai-ying |
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| Affiliation: | College of Life Science, Guizhou University, Guiyang 550025, China |
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| Abstract: | By means of degreasing, water-extracting, acetone-precipitating and dialyzing in distilled water, the total carbohydrate in the mycelial powder of Paecilomyces cicadae is tested as 34.79% by Phenol-Sulphuric Measure method and the protein content is 63.45% by Lowry. The glycoprotein possesses two absorbing peaks (PS1 and PS2) under ultraviolet spectrum as separated by Sephadex G-25. The protein content is 77.1% in PS1 and 53.02% in PS2, while the total carbohydrate is 21.11% in PS1 and 44.98% in PS2, respectively. |
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| Keywords: | Paecilomyces cicadae mycelia total carbohydrate glycoprotein column chromatography |
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