| 生物表面活性剂发酵条件的响应面分析优化 |
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| 引用本文： | 苏晓钰,曹凤麟,康宏,李盛贤. 生物表面活性剂发酵条件的响应面分析优化[J]. 油田化学, 2011, 28(4) |
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| 作者姓名： | 苏晓钰 曹凤麟 康宏 李盛贤 |
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| 作者单位： | 1. 青岛市产品质量监督检验所,山东青岛,266071
2. 黑龙江大学生命科学学院,黑龙江哈尔滨,150080 |
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| 基金项目： | 黑龙江大学校青年基金“生物表面活性剂产生菌的筛选及其在三次采油中的应用”(项目编号QL200724) |
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| 摘 要： | 本文利用Plackett-Burman设计法和响应面分析法,对产生物表面活性剂的采油菌株DN4-3的发酵条件进行优化,筛选出三个显著影响因子（葡萄糖用量、酵母浸粉用量和氯化钠用量）。在此基础上,通过最陡爬坡实验和响应面分析,研究了显著因素的最佳水平及其交互作用,确定了最佳发酵条件。最佳发酵条件为：葡萄糖27.83 g/L、酵母浸粉2.73 g/L、NaCl 2.37 g/L、KH2PO4 9 g/L、K2HPO4 3 g/L、CaCl2 0.16 g/L、MgSO4.7H2O 0.5 g/L、接种量4%、种龄16 h、装液量250 mL/500 mL、温度37℃、初始pH值7.0、摇床转速160 r/min。采用优化后条件进行摇瓶发酵,产表面活性剂1025.98 mg/L,与预测值很接近,比优化前提高了35.43%。
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| 关 键 词： | 生物表面活性剂 Plackett-Burman设计 响应面分析 微生物三次采油 |
Optimization of Fermentation Conditions of Biosurfactant by Response Surface Analysis |
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| SU Xiao-Yu,CAO Feng-Lin,KANG Hong,LI Sheng-Xian. Optimization of Fermentation Conditions of Biosurfactant by Response Surface Analysis[J]. Oilfield Chemistry, 2011, 28(4) |
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| Authors: | SU Xiao-Yu CAO Feng-Lin KANG Hong LI Sheng-Xian |
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| Affiliation: | SU Xiao-Yu1,CAO Feng-Lin2,KANG Hong2,LI Sheng-Xian2 (1.Qingdao Supervision and Testing Center of Product Quality,Qingdao,Shandong 266071,P R of China,2.College of Life Science,Heilongjiang University,Harbin,Heilongjiang 150080,P R of China) |
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| Abstract: | The fermentation conditions of biosurfactant production by strain DN4-3 were optimized with the Plackett-Burman design and response surface analysis.Plackett-Burman design was used to evaluate the influence of related factors,as a result,three significant factors,such as the content of glucose,the content of yeast extract power and NaCl content,were selected.The path of steepest ascent and response surface analysis were used to determine the optimal levels of the significant factors.Based on the single fact... |
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| Keywords: | biosurfactant Plackett-Burman design response surface analysis MEOR |
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